Imperial College London
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DrAliAbbara

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Clinical Senior Lecturer in Diabetes and Endocrinology
 
 
 
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Contact

 

+44 (0)20 3313 4843ali.abbara Website

 
 
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Location

 

6N6Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Romero-Ruiz:2019:10.1016/j.ajog.2019.01.228,
author = {Romero-Ruiz, A and Avendaño, MS and Dominguez, F and Lozoya, T and Molina-Abril, H and Sangiao-Alvarellos, S and Gurrea, M and Lara-Chica, M and Fernandez-Sanchez, M and Torres-Jimenez, E and Perdices-Lopez, C and Abbara, A and Steffani, L and Calzado, MA and Dhillo, WS and Pellicer, A and Tena-Sempere, M},
doi = {10.1016/j.ajog.2019.01.228},
journal = {American Journal of Obstetrics and Gynecology},
pages = {480.e1--480.e17},
title = {Deregulation of miR-324/KISS1/kisspeptin in early ectopic pregnancy: mechanistic findings with clinical and diagnostic implications},
url = {http://dx.doi.org/10.1016/j.ajog.2019.01.228},
volume = {220},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Ectopic pregnancy is a life-threatening condition for which novel screening tools that would enable early accurate diagnosis would improve clinical outcomes. Kisspeptins, encoded by KISS1, play an essential role in human reproduction, at least partially by regulating placental function and possibly embryo implantation. Kisspeptin levels are elevated massively in normal pregnancy and reportedly altered in various gestational pathologic diseases. Yet, the pathophysiologic role of KISS1/kisspeptin in ectopic pregnancy has not been investigated previously. OBJECTIVE: The purpose of this study was to evaluate changes of KISS1/kisspeptin levels in ectopic pregnancy and their underlaying molecular mechanisms and to ascertain the diagnostic implications of these changes. STUDY DESIGN: A total of 122 women with normal pregnancy who underwent voluntary termination of pregnancy and 84 patients who experienced tubal ectopic pregnancy were recruited. Measurements of plasma kisspeptins and KISS1 expression analyses in human embryonic/placental tissue were conducted in ectopic pregnancy and voluntary termination of pregnancy control subjects during the early gestational window (<12 weeks). Putative microRNA regulators of KISS1 were predicted in silico, followed by expression analyses of selected microRNAs and validation of repressive interactions in vitro. Circulating levels of these microRNAs were also assayed in ectopic pregnancy vs voluntary termination of pregnancy. RESULTS: Circulating kisspeptins gradually increased during the first trimester of normal pregnancy but were reduced markedly in ectopic pregnancy. This profile correlated with the expression levels of KISS1 in human embryonic/placental tissue, which increased in voluntary termination of pregnancy but remained suppressed in ectopic pregnancy. Bioinformatic predictions and expression analyses identified miR-27b-3p and miR-324-3p as putative repressors of KISS1 in human embryonic/placental tissue
AU  - Romero-Ruiz,A
AU  - Avendaño,MS
AU  - Dominguez,F
AU  - Lozoya,T
AU  - Molina-Abril,H
AU  - Sangiao-Alvarellos,S
AU  - Gurrea,M
AU  - Lara-Chica,M
AU  - Fernandez-Sanchez,M
AU  - Torres-Jimenez,E
AU  - Perdices-Lopez,C
AU  - Abbara,A
AU  - Steffani,L
AU  - Calzado,MA
AU  - Dhillo,WS
AU  - Pellicer,A
AU  - Tena-Sempere,M
DO  - 10.1016/j.ajog.2019.01.228
EP  - 1
PY  - 2019///
SN  - 0002-9378
SP  - 480
TI  - Deregulation of miR-324/KISS1/kisspeptin in early ectopic pregnancy: mechanistic findings with clinical and diagnostic implications
T2  - American Journal of Obstetrics and Gynecology
UR  - http://dx.doi.org/10.1016/j.ajog.2019.01.228
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30707968
UR  - https://www.sciencedirect.com/science/article/pii/S0002937819302820?via%3Dihub
UR  - http://hdl.handle.net/10044/1/69020
VL  - 220
ER  -
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