Imperial College London
Alternate

ProfessorAlisonHolmes

Faculty of Medicine, Department of Infectious Disease

Professor of Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 3313 1283alison.holmes

 
 
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Location

 

8N16Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Freeman:2023:10.1021/acssensors.2c02780,
author = {Freeman, DME and Ming, DK and Wilson, R and Herzog, PL and Schulz, C and Felice, AKG and Chen, Y-C and O'Hare, D and Holmes, AH and Cass, AEG},
doi = {10.1021/acssensors.2c02780},
journal = {ACS Sensors},
pages = {1639--1647},
title = {Continuous measurement of lactate concentration in human subjects through direct electron transfer from enzymes to microneedle electrodes},
url = {http://dx.doi.org/10.1021/acssensors.2c02780},
volume = {8},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Microneedle lactate sensors may be used to continuously measure lactate concentration in the interstitial fluid in a minimally invasive and pain-free manner. First- and second-generation enzymatic sensors produce a redox-active product that is electrochemically sensed at the electrode surface. Direct electron transfer enzymes produce electrons directly as the product of enzymatic action; in this study, a direct electron transfer enzyme specific to lactate has been immobilized onto a microneedle surface to create lactate-sensing devices that function at low applied voltages (0.2 V). These devices have been validated in a small study of human volunteers; lactate concentrations were raised and lowered through physical exercise and subsequent rest. Lactazyme microneedle devices show good agreement with concurrently obtained and analyzed serum lactate levels.
AU  - Freeman,DME
AU  - Ming,DK
AU  - Wilson,R
AU  - Herzog,PL
AU  - Schulz,C
AU  - Felice,AKG
AU  - Chen,Y-C
AU  - O'Hare,D
AU  - Holmes,AH
AU  - Cass,AEG
DO  - 10.1021/acssensors.2c02780
EP  - 1647
PY  - 2023///
SN  - 2379-3694
SP  - 1639
TI  - Continuous measurement of lactate concentration in human subjects through direct electron transfer from enzymes to microneedle electrodes
T2  - ACS Sensors
UR  - http://dx.doi.org/10.1021/acssensors.2c02780
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000959722300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://pubs.acs.org/doi/10.1021/acssensors.2c02780
UR  - http://hdl.handle.net/10044/1/110008
VL  - 8
ER  -
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