Imperial College London

Professor Dame Amanda Fisher

Faculty of MedicineInstitute of Clinical Sciences

Head of the Institute of Clinical Sciences
 
 
 
//

Contact

 

amanda.fisher

 
 
//

Assistant

 

Ms Alessandra Lisini +44 (0)20 3313 8236

 
//

Location

 

CRB (Clinical Research Building)Hammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Chiu:2016:10.1002/jctb.4803,
author = {Chiu, FWY and Bagci, H and Fisher, AG and deMello, AJ and Elvira, KS},
doi = {10.1002/jctb.4803},
journal = {Journal of Chemical Technology and Biotechnology},
pages = {16--24},
title = {A microfluidic toolbox for cell fusion},
url = {http://dx.doi.org/10.1002/jctb.4803},
volume = {91},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Cellular fusion is a key process in many fields ranging from historical gene mapping studies and monoclonal antibody production, through to cell reprogramming. Traditional methodologies for cell fusion rely on the random pairing of different cell types and generally result in low and variable fusion efficiencies. These approaches become particularly limiting where substantial numbers of bespoke one-to-one fusions are required, for example, for in-depth studies of nuclear reprogramming mechanisms. In recent years, microfluidic technologies have proven valuable in creating platforms where the manipulation of single cells is highly efficient, rapid and controllable. These technologies also allow the integration of different experimental steps and characterisation processes into a single platform. Although the application of microfluidic methodologies to cell fusion studies is promising, current technologies that rely on static trapping are limited both in terms of the overall number of fused cells produced and their experimental accessibility. Here we review some of the most exciting breakthroughs in core microfluidic technologies that will allow the creation of integrated platforms for controlled cell fusion at high throughput. © 2015 Society of Chemical Industry.
AU - Chiu,FWY
AU - Bagci,H
AU - Fisher,AG
AU - deMello,AJ
AU - Elvira,KS
DO - 10.1002/jctb.4803
EP - 24
PY - 2016///
SN - 1097-4660
SP - 16
TI - A microfluidic toolbox for cell fusion
T2 - Journal of Chemical Technology and Biotechnology
UR - http://dx.doi.org/10.1002/jctb.4803
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000367951400003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/32772
VL - 91
ER -