Imperial College London

Professor Dame Amanda Fisher

Faculty of MedicineInstitute of Clinical Sciences

Head of the Institute of Clinical Sciences







Ms Alessandra Lisini +44 (0)20 3313 8236




CRB (Clinical Research Building)Hammersmith Campus






BibTex format

author = {Graham, B and Marcais, A and Dharmalingam, G and Carroll, T and Kanellopoulou, C and Graumann, J and Nesterova, TB and Bermange, A and Brazauskas, P and Xella, B and Kriaucionis, S and Higgs, DR and Brockdorff, N and Mann, M and Fisher, AG and Merkenschlager, M},
doi = {10.1016/j.stemcr.2016.03.005},
journal = {Stem Cell Reports},
pages = {635--642},
title = {MicroRNAs of the miR-290-295 Family Maintain Bivalency in Mouse Embryonic Stem Cells.},
url = {},
volume = {6},
year = {2016}

RIS format (EndNote, RefMan)

AB - Numerous developmentally regulated genes in mouse embryonic stem cells (ESCs) are marked by both active (H3K4me3)- and polycomb group (PcG)-mediated repressive (H3K27me3) histone modifications. This bivalent state is thought to be important for transcriptional poising, but the mechanisms that regulate bivalent genes and the bivalent state remain incompletely understood. Examining the contribution of microRNAs (miRNAs) to the regulation of bivalent genes, we found that the miRNA biogenesis enzyme DICER was required for the binding of the PRC2 core components EZH2 and SUZ12, and for the presence of the PRC2-mediated histone modification H3K27me3 at many bivalent genes. Genes that lost bivalency were preferentially upregulated at the mRNA and protein levels. Finally, reconstituting Dicer-deficient ESCs with ESC miRNAs restored bivalent gene repression and PRC2 binding at formerly bivalent genes. Therefore, miRNAs regulate bivalent genes and the bivalent state itself.
AU - Graham,B
AU - Marcais,A
AU - Dharmalingam,G
AU - Carroll,T
AU - Kanellopoulou,C
AU - Graumann,J
AU - Nesterova,TB
AU - Bermange,A
AU - Brazauskas,P
AU - Xella,B
AU - Kriaucionis,S
AU - Higgs,DR
AU - Brockdorff,N
AU - Mann,M
AU - Fisher,AG
AU - Merkenschlager,M
DO - 10.1016/j.stemcr.2016.03.005
EP - 642
PY - 2016///
SN - 2213-6711
SP - 635
TI - MicroRNAs of the miR-290-295 Family Maintain Bivalency in Mouse Embryonic Stem Cells.
T2 - Stem Cell Reports
UR -
UR -
VL - 6
ER -