Imperial College London

Professor Dame Amanda Fisher

Faculty of MedicineInstitute of Clinical Sciences

Head of the Institute of Clinical Sciences
 
 
 
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Contact

 

amanda.fisher

 
 
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Assistant

 

Ms Alessandra Lisini +44 (0)20 3313 8236

 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Fisher:2016:10.1038/ncomms12354,
author = {Fisher, AG and Cantone, I and Bagci, H and Dormann, D and Dharmalingam, G and Nesterova, T and Brockdorff, N and Rougeulle, C and Vallot, C and Heard, E and Chaligne, R and Merkenschlager, M and Fisher, AG},
doi = {10.1038/ncomms12354},
journal = {Nature Communications},
title = {Ordered chromatin changes and human X chromosome reactivation by cell fusion-mediated pluripotent reprogramming},
url = {http://dx.doi.org/10.1038/ncomms12354},
volume = {7},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Erasure of epigenetic memory is required to convert somatic cells towards pluripotency. Reactivation of the inactive X chromosome (Xi) has been used to model epigenetic reprogramming in mouse, but human studies are hampered by Xi epigenetic instability and difficulties in tracking partially reprogrammed iPSCs. Here we use cell fusion to examine the earliest events in the reprogramming-induced Xi reactivation of human female fibroblasts. We show that a rapid and widespread loss of Xi-associated H3K27me3 and XIST occurs in fused cells and precedes the bi-allelic expression of selected Xi-genes by many heterokaryons (30-50%). After cell division, RNA-FISH and RNA-seq analyses confirm that Xi reactivation remains partial and that induction of human pluripotency-specific XACT transcripts is rare (1%). These data effectively separate pre- and post-mitotic events in reprogramming-induced Xi reactivation and reveal a complex hierarchy of epigenetic changes that are required to reactivate the genes on the human Xi chromosome.
AU - Fisher,AG
AU - Cantone,I
AU - Bagci,H
AU - Dormann,D
AU - Dharmalingam,G
AU - Nesterova,T
AU - Brockdorff,N
AU - Rougeulle,C
AU - Vallot,C
AU - Heard,E
AU - Chaligne,R
AU - Merkenschlager,M
AU - Fisher,AG
DO - 10.1038/ncomms12354
PY - 2016///
SN - 2041-1723
TI - Ordered chromatin changes and human X chromosome reactivation by cell fusion-mediated pluripotent reprogramming
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/ncomms12354
UR - http://hdl.handle.net/10044/1/39065
VL - 7
ER -