Imperial College London

Professor DameMandyFisher

Faculty of MedicineInstitute of Clinical Sciences

Head of the Institute of Clinical Sciences
 
 
 
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Contact

 

+44 (0)20 3313 8236amanda.fisher

 
 
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Assistant

 

Ms Alessandra Lisini +44 (0)20 3313 8236

 
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Location

 

CRB (Clinical Research Building)Hammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Malinowski:2016:10.1007/978-1-4939-6380-5_25,
author = {Malinowski, AR and Fisher, AG},
doi = {10.1007/978-1-4939-6380-5_25},
pages = {289--299},
title = {Reprogramming of Somatic Cells Towards Pluripotency by Cell Fusion.},
url = {http://dx.doi.org/10.1007/978-1-4939-6380-5_25},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - CHAP
AB - Pluripotent reprogramming can be dominantly induced in a somatic nucleus upon fusion with a pluripotent cell such as embryonic stem (ES) cell. Cell fusion between ES cells and somatic cells results in the formation of heterokaryons, in which the somatic nuclei begin to acquire features of the pluripotent partner. The generation of interspecies heterokaryons between mouse ES- and human somatic cells allows an experimenter to distinguish the nuclear events occurring specifically within the reprogrammed nucleus. Therefore, cell fusion provides a simple and rapid approach to look at the early nuclear events underlying pluripotent reprogramming. Here, we describe a polyethylene glycol (PEG)-mediated cell fusion protocol to generate interspecies heterokaryons and intraspecies hybrids between ES cells and B lymphocytes or fibroblasts.
AU - Malinowski,AR
AU - Fisher,AG
DO - 10.1007/978-1-4939-6380-5_25
EP - 299
PY - 2016///
SP - 289
TI - Reprogramming of Somatic Cells Towards Pluripotency by Cell Fusion.
UR - http://dx.doi.org/10.1007/978-1-4939-6380-5_25
UR - https://www.ncbi.nlm.nih.gov/pubmed/27659994
ER -