Publications
138 results found
Brown KE, Guest SS, Smale ST, et al., 1997, Association of transcriptionally silent genes with Ikaros complexes at centromeric heterochromatin, CELL, Vol: 91, Pages: 845-854, ISSN: 0092-8674
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- Citations: 648
Merkenschlager M, Graf D, Lovatt M, et al., 1997, How many thymocytes audition for selection?, Journal of Experimental Medicine, Vol: 186, Pages: 1149-1158, ISSN: 0022-1007
T cell maturation requires the rearrangement of clonotypic T cell receptors (TCR) capable of interacting with major histocompatibility complex (MHC) ligands to initiate positive and negative selection. Only 3–5% of thymocytes mature to join the peripheral T cell pool. To investigate the basis for this low success rate, we have measured the frequency of preselection thymocytes capable of responding to MHC. As many as one in five MHC-naive thymocytes show upregulation of activation markers on exposure to MHC-expressing thymic stroma in short-term reaggregate culture. The majority of these cells display physiological changes consistent with entry into the selection process within 24 h. By exposing TCR transgenic thymocytes to a range of MHC–peptide complexes, we show that CD69 induction is indicative of thymocyte selection, positive or negative. Our data provide evidence that the fraction of thymocytes that qualify to enter the thymic selection process far exceeds the fraction that successfully complete it, and suggest that most MHC-reactive thymocytes are actively eliminated in the course of selection.
Graf D, Fisher AG, Merkenschlager M, 1997, Rational primer design greatly improves differential display PCR (DD-PCR), NUCLEIC ACIDS RESEARCH, Vol: 25, Pages: 2239-2240, ISSN: 0305-1048
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- Citations: 29
FISHER AG, BURDET C, BUNCE C, et al., 1995, LYMPHOPROLIFERATIVE DISORDERS IN IL-7 TRANSGENIC MICE - EXPANSION OF IMMATURE B-CELLS WHICH RETAIN MACROPHAGE POTENTIAL, INTERNATIONAL IMMUNOLOGY, Vol: 7, Pages: 415-423, ISSN: 0953-8178
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- Citations: 62
LOMBARDPLATET S, FISHER AG, MEYER V, et al., 1995, EXPRESSION OF FUNCTIONAL MHC CLASS-II MOLECULES BY A MOUSE PRO-B CELL CLONE, DEVELOPMENTAL IMMUNOLOGY, Vol: 4, Pages: 85-92, ISSN: 1044-6672
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- Citations: 5
FISHER AG, WALTZINGER C, CEREDIG R, 1992, SELECTION OF MURINE T-CELL RECEPTOR ALPHA-BETA AND GAMMA-DELTA CELLS IN ORGAN-CULTURES ESTABLISHED FROM 14-DAY EMBRYOS, EUROPEAN JOURNAL OF IMMUNOLOGY, Vol: 22, Pages: 1765-1771, ISSN: 0014-2980
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- Citations: 5
LARSSON L, HICKS R, FISHER AG, 1992, IDENTIFICATION OF MURINE THYMOCYTE POPULATIONS CAPABLE OF EXTENSIVE PROLIFERATION IN SERIALLY PASSAGED THYMIC ORGAN-CULTURES, THYMUS, Vol: 19, Pages: 193-206, ISSN: 0165-6090
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- Citations: 1
MERKENSCHLAGER M, FISHER AG, 1992, SELECTIVE MANIPULATION OF THE HUMAN T-CELL RECEPTOR REPERTOIRE EXPRESSED BY THYMOCYTES IN ORGAN-CULTURE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 89, Pages: 4255-4259, ISSN: 0027-8424
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- Citations: 13
MERKENSCHLAGER M, FISHER AG, 1992, HUMAN POSTNATAL THYMOCYTES GENERATE PHENOTYPICALLY IMMATURE CD3DIM, CD5DIM, CD1ABRIGHT PROGENY IN ORGAN-CULTURE, JOURNAL OF IMMUNOLOGY, Vol: 148, Pages: 1012-1015, ISSN: 0022-1767
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- Citations: 18
FISHER AG, CEREDIG R, 1991, GAMMA-DELTA-T-CELLS EXPRESSING CD8 OR CD4LOW APPEAR EARLY IN MURINE FETAL THYMUS DEVELOPMENT, INTERNATIONAL IMMUNOLOGY, Vol: 3, Pages: 1323-1328, ISSN: 0953-8178
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- Citations: 32
DANIELS RS, SMITH MH, FISHER AG, 1991, MOLECULAR CHARACTERIZATION OF BIOLOGICALLY DIVERSE ENVELOPE VARIANTS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 DERIVED FROM AN INDIVIDUAL, JOURNAL OF VIROLOGY, Vol: 65, Pages: 5574-5578, ISSN: 0022-538X
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- Citations: 6
MERKENSCHLAGER M, IKEDA H, WILKINSON D, et al., 1991, ALLORECOGNITION OF HLA-DR AND HLA-DQ TRANSFECTANTS BY HUMAN CD45RA AND CD45R0 CD4 T-CELLS - REPERTOIRE ANALYSIS AND ACTIVATION REQUIREMENTS, EUROPEAN JOURNAL OF IMMUNOLOGY, Vol: 21, Pages: 79-88, ISSN: 0014-2980
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- Citations: 26
MERKENSCHLAGER M, FISHER AG, 1991, CD45 ISOFORM SWITCHING PRECEDES THE ACTIVATION-DRIVEN DEATH OF HUMAN THYMOCYTES BY APOPTOSIS, INTERNATIONAL IMMUNOLOGY, Vol: 3, Pages: 1-7, ISSN: 0953-8178
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- Citations: 59
GOFF LK, LARSSON L, FISHER AG, 1990, EXPRESSION OF HIGH-MOLECULAR-WEIGHT ISOFORMS OF CD45 BY MOUSE THYMIC PROGENITOR CELLS, EUROPEAN JOURNAL OF IMMUNOLOGY, Vol: 20, Pages: 665-671, ISSN: 0014-2980
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- Citations: 29
FISHER AG, SMITH MH, MOORE MM, et al., 1990, DEFECTIVE VIRUSES IN AIDS, AIDS RESEARCH AND HUMAN RETROVIRUSES, Vol: 6, Pages: 56-57, ISSN: 0889-2229
FISHER AG, GOFF LK, LIGHTSTONE L, et al., 1989, PROBLEMS IN THE PHYSIOLOGY OF CLASS-I AND CLASS-II MHC MOLECULES, AND OF CD45, 3RD SYMP ON IMMUNOLOGICAL RECOGNITION, Publisher: COLD SPRING HARBOR LABORATORY PRESS, Pages: 667-674
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- Citations: 9
FISHER AG, GOFF LK, LIGHTSTONE L, et al., 1989, PROBLEMS IN THE PHYSIOLOGY OF CLASS-I AND CLASS-II MHC MOLECULES, AND OF CD45, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, Vol: 54, Pages: 667-674, ISSN: 0091-7451
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- Citations: 9
Fisher AG, Goff LK, Lightstone L, et al., 1989, Problems in the physiology of class I and class II MHC molecules, and of CD45., Cold Spring Harb Symp Quant Biol, Vol: 54 Pt 2, Pages: 667-674, ISSN: 0091-7451
1. Co-processing of alloantigens suggests that epitope-loaded MHC class I molecules may pass from tissue cells to dendritic cells. 2. Antigen-presenting cells in the thymus need some special trick in order to load their MHC class II molecules with epitopes from "intermediate concentration" self-proteins in order to induce self-tolerance in developing cells. 3. Cell-cell interactions may transmit signals simply by rearranging surface glycoproteins and thus locally perturbing a phosphorylation equilibrium. 4. The CD45 and STB1 phenotype of most cells in the thymus may be characteristic of a doomed cell.
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