Imperial College London
ANAT MELAMED

ANAT MELAMED

Faculty of Medicine, Department of Infectious Disease

Research Associate
 
 
 
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Contact

 

anat.melamed07

 
 
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Location

 

VD1Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Melamed:2018:10.1101/277335,
author = {Melamed, A and Yaguchi, H and Miura, M and Witkover, A and Fitzgerald, TW and Birney, E and Bangham, CRM},
doi = {10.1101/277335},
title = {The human leukemia virus HTLV-1 alters the structure and transcription of host chromatin <i>in cis</i>},
url = {http://dx.doi.org/10.1101/277335},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>Chromatin looping controls gene expression by regulating promoter-enhancer contacts, the spread of epigenetic modifications, and the segregation of the genome into transcriptionally active and inactive compartments. We studied the impact on the structure and expression of host chromatin by the human retrovirus HTLV-1. We show that HTLV-1 disrupts host chromatin structure by forming loops between the provirus and the host genome; certain loops depend on the critical chromatin architectural protein CTCF, which we recently showed binds to the HTLV-1 provirus. Finally, we show that the provirus causes two distinct patterns of abnormal transcription of the host genome <jats:italic>in cis</jats:italic>: bidirectional transcription in the host genome immediately flanking the provirus, and clone-specific transcription <jats:italic>in cis</jats:italic> at non-contiguous loci up to >300 kb from the integration site. We conclude that HTLV-1 causes insertional mutagenesis up to the megabase range in the host genome in >10<jats:sup>4</jats:sup> persistently-maintained HTLV-1<jats:sup>+</jats:sup> T-cell clones in vivo.</jats:p>
AU  - Melamed,A
AU  - Yaguchi,H
AU  - Miura,M
AU  - Witkover,A
AU  - Fitzgerald,TW
AU  - Birney,E
AU  - Bangham,CRM
DO  - 10.1101/277335
PY  - 2018///
TI  - The human leukemia virus HTLV-1 alters the structure and transcription of host chromatin <i>in cis</i>
UR  - http://dx.doi.org/10.1101/277335
UR  - http://hdl.handle.net/10044/1/64461
ER  -
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