Imperial College London
ANAT MELAMED

ANAT MELAMED

Faculty of Medicine, Department of Infectious Disease

Research Associate
 
 
 
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Contact

 

anat.melamed07

 
 
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Location

 

VD1Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Izaki:2021:10.1371/journal.ppat.1009271,
author = {Izaki, M and Yasunaga, J-I and Nosaka, K and Sugata, K and Utsunomiya, H and Suehiro, Y and Shichijo, T and Yamada, A and Sugawara, Y and Hibi, T and Inomata, Y and Akari, H and Melamed, A and Bangham, C and Matsuoka, M},
doi = {10.1371/journal.ppat.1009271},
journal = {PLoS Pathogens},
title = {In vivo dynamics and adaptation of HTLV-1-infected clones under different clinical conditions},
url = {http://dx.doi.org/10.1371/journal.ppat.1009271},
volume = {17},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Human T-cell leukemia virus type 1 (HTLV-1) spreads through cell contact. Therefore, this virus persists and propagates within the host by two routes: clonal proliferation of infected cells and de novo infection. The proliferation is influenced by the host immune responses and expression of viral genes. However, the detailed mechanisms that control clonal expansion of infected cells remain to be elucidated. In this study, we show that newly infected clones were strongly suppressed, and then stable clones were selected, in a patient who was infected by live liver transplantation from a seropositive donor. Conversely, most HTLV-1+ clones persisted in patients who received hematopoietic stem cell transplantation from seropositive donors. To clarify the role of cell-mediated immunity in this clonal selection, we suppressed CD8+ or CD16+ cells in simian T-cell leukemia virus type 1 (STLV-1)-infected Japanese macaques. Decreasing CD8+ T cells had marginal effects on proviral load (PVL). However, the clonality of infected cells changed after depletion of CD8+ T cells. Consistent with this, PVL at 24 hours in vitro culture increased, suggesting that infected cells with higher proliferative ability increased. Analyses of provirus in a patient who received Tax-peptide pulsed dendritic cells indicate that enhanced anti-Tax immunity did not result in a decreased PVL although it inhibited recurrence of ATL. We postulate that in vivo selection, due to the immune response, cytopathic effects of HTLV-1 and intrinsic attributes of infected cells, results in the emergence of clones of HTLV-1-infected T cells that proliferate with minimized HTLV-1 antigen expression.
AU  - Izaki,M
AU  - Yasunaga,J-I
AU  - Nosaka,K
AU  - Sugata,K
AU  - Utsunomiya,H
AU  - Suehiro,Y
AU  - Shichijo,T
AU  - Yamada,A
AU  - Sugawara,Y
AU  - Hibi,T
AU  - Inomata,Y
AU  - Akari,H
AU  - Melamed,A
AU  - Bangham,C
AU  - Matsuoka,M
DO  - 10.1371/journal.ppat.1009271
PY  - 2021///
SN  - 1553-7366
TI  - In vivo dynamics and adaptation of HTLV-1-infected clones under different clinical conditions
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1009271
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000616412900010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009271
UR  - http://hdl.handle.net/10044/1/109373
VL  - 17
ER  -
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