Imperial College London
ANAT MELAMED

ANAT MELAMED

Faculty of Medicine, Department of Infectious Disease

Research Associate
 
 
 
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Contact

 

anat.melamed07

 
 
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Location

 

VD1Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cook:2016:10.1186/s12977-015-0236-7,
author = {Cook, LBM and Melamed, A and Demontis, MA and Laydon, D and Martin, F and Fox, J and Tosswill, J and De, Freitas D and Price, A and Medcalf, J and Neuberger, J and Bangham, C and Taylor, G},
doi = {10.1186/s12977-015-0236-7},
journal = {Retrovirology},
title = {Rapid dissemination of human T-lymphotropic virus type 1 during primary infection in transplant recipients},
url = {http://dx.doi.org/10.1186/s12977-015-0236-7},
volume = {13},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundHuman T-lymphotropic virus type 1 (HTLV-1) infects an estimated 10 million persons globally with transmission resulting in lifelong infection. Disease, linked to high proviral load, occurs in a minority. In established infection HTLV-1 replicates through infectious spread and clonal expansion of infected lymphocytes. Little is known about acute HTLV-1 infection. The kinetics of early HTLV-1 infection, following transplantation-acquired infection in three recipients from one HTLV-1 infected donor, is reported. The recipients were treated with two HTLV-1 enzyme inhibitors 3 weeks post exposure following the detection of HTLV-1 provirus at low level in each recipient. HTLV-1 infection was serially monitored by serology, quantification of proviral load and HTLV-1 2LTR DNA circles and by HTLV-1 unique integration site analysis.ResultsHTLV-1 antibodies were first detected 16–39 days post-transplantation. HTLV-1 provirus was detected by PCR on day 16–23 and increased by 2–3 log by day 38–45 with a peak proviral doubling time of 1.4 days, after which steady state was reached. The rapid proviral load expansion was associated with high frequency of HTLV-1 2LTR DNA circles. The number of HTLV-1 unique integration sites was high compared with established HTLV-1 infection. Clonal expansion of infected cells was detected as early as day 37 with high initial oligoclonality index, consistent with early mitotic proliferation.ConclusionsIn recipients infected through organ transplantation HTLV-1 disseminated rapidly despite early anti-HTLV-1 treatment. Proviral load set point was reached within 6 weeks. Seroconversion was not delayed. Unique integration site analysis and HTLV-1 2LTR DNA circles indicated early clonal expansion and high rate of infectious spread.
AU  - Cook,LBM
AU  - Melamed,A
AU  - Demontis,MA
AU  - Laydon,D
AU  - Martin,F
AU  - Fox,J
AU  - Tosswill,J
AU  - De,Freitas D
AU  - Price,A
AU  - Medcalf,J
AU  - Neuberger,J
AU  - Bangham,C
AU  - Taylor,G
DO  - 10.1186/s12977-015-0236-7
PY  - 2016///
SN  - 1742-4690
TI  - Rapid dissemination of human T-lymphotropic virus type 1 during primary infection in transplant recipients
T2  - Retrovirology
UR  - http://dx.doi.org/10.1186/s12977-015-0236-7
UR  - http://hdl.handle.net/10044/1/28548
VL  - 13
ER  -
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