Imperial College London
ANAT MELAMED

ANAT MELAMED

Faculty of Medicine, Department of Infectious Disease

Research Associate
 
 
 
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anat.melamed07

 
 
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Location

 

VD1Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Satou:2016:10.1073/pnas.1423199113,
author = {Satou, Y and Miyazato, P and Ishihara, Y and Yaguchi, H and Melamed, A and Miura, M and Fukuda, A and Nosaka, K and Watanabe, T and Rowan, A and Nakao, M and Bangham, C},
doi = {10.1073/pnas.1423199113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
pages = {3054--3059},
title = {The retrovirus HTLV-1 inserts an ectopic CTCF-binding site into the human genome},
url = {http://dx.doi.org/10.1073/pnas.1423199113},
volume = {113},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Human  T-lymphotropic  virus  type  1  (HTLV-1)  is  a  retrovirus  thatcauses malignant and inflammatory diseases in 10% of infectedpeople. A typical host has between 104and 105clones of HTLV-1-infected T lymphocytes, each clone distinguished by the genomicintegration site of the single-copy HTLV-1 provirus. TheHBZgeneis constitutively expressed from the minus strand of the provirus,whereas plus-strand expression, required for viral propagation touninfected  cells,  is  suppressed  or  intermittentin  vivo,  allowingescape from host immune surveillance. It remains unknown whatregulates this pattern of proviral transcription and latency. Herewe show that CTCF, a key regulator of chromatin structure andfunction,  binds  to  the  provirus  at  a  sharp  border  in  epigeneticmodifications in the pX region of the HTLV-1 provirus, in T cellsnaturally infected with HTLV-1. CTCF is a zinc-finger protein thatbinds to an insulator region in genomic DNA and plays a funda-mental  role  in  controlling  higher-order  chromatin  structure  andgene  expression  in  vertebrate  cells.  We  show  that  CTCF  boundto  HTLV-1  acts  as  an  enhancer  blocker,  regulates  HTLV-1  mRNAsplicing, and forms long-distance interactions with flanking hostchromatin.  CTCF  binding  sites  have  been  propagated  through-out the genome by transposons in certain primate lineages, butCTCF  binding  has  not  previously  been  described  in  present-dayexogenous retroviruses. The presence of an ectopic CTCF bindingsite introduced by the retrovirus in tens of thousands of genomiclocations has the potential to cause widespread abnormalities inhost cell chromatin structure and gene expression.
AU  - Satou,Y
AU  - Miyazato,P
AU  - Ishihara,Y
AU  - Yaguchi,H
AU  - Melamed,A
AU  - Miura,M
AU  - Fukuda,A
AU  - Nosaka,K
AU  - Watanabe,T
AU  - Rowan,A
AU  - Nakao,M
AU  - Bangham,C
DO  - 10.1073/pnas.1423199113
EP  - 3059
PY  - 2016///
SN  - 0027-8424
SP  - 3054
TI  - The retrovirus HTLV-1 inserts an ectopic CTCF-binding site into the human genome
T2  - Proceedings of the National Academy of Sciences of the United States of America
UR  - http://dx.doi.org/10.1073/pnas.1423199113
UR  - https://www.pnas.org/content/113/11/3054/tab-article-info
UR  - http://hdl.handle.net/10044/1/29817
VL  - 113
ER  -
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