Imperial College London
ANAT MELAMED

ANAT MELAMED

Faculty of Medicine, Department of Infectious Disease

Research Associate
 
 
 
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Contact

 

anat.melamed07

 
 
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Location

 

VD1Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rowan:2016:10.1371/journal.ppat.1006030,
author = {Rowan, AG and Witkover, A and Melamed, A and Tanaka, Y and Cook, LBM and Fields, P and Taylor, GP and Bangham, CRM},
doi = {10.1371/journal.ppat.1006030},
journal = {PLoS Pathog},
title = {T Cell Receptor Vβ Staining Identifies the Malignant Clone in Adult T cell Leukemia and Reveals Killing of Leukemia Cells by Autologous CD8+ T cells.},
url = {http://dx.doi.org/10.1371/journal.ppat.1006030},
volume = {12},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - There is growing evidence that CD8+ cytotoxic T lymphocyte (CTL) responses can contribute to long-term remission of many malignancies. The etiological agent of adult T-cell leukemia/lymphoma (ATL), human T lymphotropic virus type-1 (HTLV-1), contains highly immunogenic CTL epitopes, but ATL patients typically have low frequencies of cytokine-producing HTLV-1-specific CD8+ cells in the circulation. It remains unclear whether patients with ATL possess CTLs that can kill the malignant HTLV-1 infected clone. Here we used flow cytometric staining of TCRVβ and cell adhesion molecule-1 (CADM1) to identify monoclonal populations of HTLV-1-infected T cells in the peripheral blood of patients with ATL. Thus, we quantified the rate of CD8+-mediated killing of the putative malignant clone in ex vivo blood samples. We observed that CD8+ cells from ATL patients were unable to lyse autologous ATL clones when tested directly ex vivo. However, short in vitro culture restored the ability of CD8+ cells to kill ex vivo ATL clones in some donors. The capacity of CD8+ cells to lyse HTLV-1 infected cells which expressed the viral sense strand gene products was significantly enhanced after in vitro culture, and donors with an ATL clone that expressed the HTLV-1 Tax gene were most likely to make a detectable lytic CD8+ response to the ATL cells. We conclude that some patients with ATL possess functional tumour-specific CTLs which could be exploited to contribute to control of the disease.
AU  - Rowan,AG
AU  - Witkover,A
AU  - Melamed,A
AU  - Tanaka,Y
AU  - Cook,LBM
AU  - Fields,P
AU  - Taylor,GP
AU  - Bangham,CRM
DO  - 10.1371/journal.ppat.1006030
PY  - 2016///
TI  - T Cell Receptor Vβ Staining Identifies the Malignant Clone in Adult T cell Leukemia and Reveals Killing of Leukemia Cells by Autologous CD8+ T cells.
T2  - PLoS Pathog
UR  - http://dx.doi.org/10.1371/journal.ppat.1006030
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27893842
UR  - http://hdl.handle.net/10044/1/42951
VL  - 12
ER  -
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