Imperial College London
Alternate

Emeritus ProfessorAndrewHolmes

Faculty of Natural Sciences, Department of Chemistry

Distinguished Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 9286andrew.holmes Website

 
 
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Assistant

 

Ms Maria Tortelli +44 (0)20 7594 9286

 
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Location

 

Molecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gregory:2016:10.1055/s-0035-1560381,
author = {Gregory, M and Catimel, B and Yin, MX and Condron, M and Burgess, AW and Holmes, AB},
doi = {10.1055/s-0035-1560381},
journal = {Synlett},
pages = {121--125},
title = {Synthesis of a Tethered myo -Inositol (1,3,4,5,6)Pentakisphosphate (IP5) Derivative as a Probe for Biological Studies},
url = {http://dx.doi.org/10.1055/s-0035-1560381},
volume = {27},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - There is sufficient evidence to suggest that myo-inositol pentakisphosphate is a vital intermediate species in higher inositol phosphate metabolism, however, its biological roles and physiological function in cells remain uncertain. A tethered myo-inositol pentakisphosphate (IP5) derivative with a terminal amine group is synthesised allowing facilitated immobilisation onto M-270 magnetic Dynabeads for pull-down experiments and biosensor chip preparation for surface plasmon resonance studies. The probes are validated by both pull-down and surface plasmon resonance (SPR) studies of the known binding protein GRP-1 (general receptor for phosphoinositides 1), and furthermore by SPR studies of protein kinase B (PKB or AKT) binding.
AU  - Gregory,M
AU  - Catimel,B
AU  - Yin,MX
AU  - Condron,M
AU  - Burgess,AW
AU  - Holmes,AB
DO  - 10.1055/s-0035-1560381
EP  - 125
PY  - 2016///
SN  - 0936-5214
SP  - 121
TI  - Synthesis of a Tethered myo -Inositol (1,3,4,5,6)Pentakisphosphate (IP5) Derivative as a Probe for Biological Studies
T2  - Synlett
UR  - http://dx.doi.org/10.1055/s-0035-1560381
VL  - 27
ER  -
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