Imperial College London
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DrAnikaSinganayagam

Faculty of Medicine, Department of Infectious Disease

Clinical Lecturer
 
 
 
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Contact

 

anika.singanayagam

 
 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Singanayagam:2020:10.1371/journal.ppat.1008362,
author = {Singanayagam, A and Zhou, J and Elderfield, RA and Frise, R and Ashcroft, J and Galiano, M and Miah, S and Nicolaou, L and Barclay, WS},
doi = {10.1371/journal.ppat.1008362},
journal = {PLoS Pathogens},
pages = {1--21},
title = {Characterising viable virus from air exhaled by H1N1 influenza-infected ferrets reveals the importance of haemagglutinin stability for airborne infectivity},
url = {http://dx.doi.org/10.1371/journal.ppat.1008362},
volume = {16},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The transmissibility and pandemic potential of influenza viruses depends on their ability to efficiently replicate and be released from an infected host, retain viability as they pass through the environment, and then initiate infection in the next host. There is a significant gap in knowledge about viral properties that enable survival of influenza viruses between hosts, due to a lack of experimental methods to reliably isolate viable virus from the air. Using a novel technique, we isolate and characterise infectious virus from droplets emitted by 2009 pandemic H1N1-infected ferrets. We demonstrate that infectious virus is predominantly released early after infection. A virus containing a mutation destabilising the haemagglutinin (HA) surface protein displayed reduced survival in air. Infectious virus recovered from droplets exhaled by ferrets inoculated with this virus contained mutations that conferred restabilisation of HA, indicating the importance of influenza HA stability for between-host survival. Using this unique approach can improve knowledge about the determinants and mechanisms of influenza transmissibility and ultimately could be applied to studies of airborne virus exhaled from infected people.
AU  - Singanayagam,A
AU  - Zhou,J
AU  - Elderfield,RA
AU  - Frise,R
AU  - Ashcroft,J
AU  - Galiano,M
AU  - Miah,S
AU  - Nicolaou,L
AU  - Barclay,WS
DO  - 10.1371/journal.ppat.1008362
EP  - 21
PY  - 2020///
SN  - 1553-7366
SP  - 1
TI  - Characterising viable virus from air exhaled by H1N1 influenza-infected ferrets reveals the importance of haemagglutinin stability for airborne infectivity
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1008362
UR  - https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008362
UR  - http://hdl.handle.net/10044/1/77051
VL  - 16
ER  -
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