Imperial College London

ProfessorBernadetteByrne

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Molecular Membrane Biology
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ehsan:2018:10.1002/cbic.201800106,
author = {Ehsan, M and Das, M and Stern, V and Du, Y and Mortensen, JS and Hariharan, P and Byrne, B and Loland, CJ and Kobilka, BK and Guan, L and Chae, PS},
doi = {10.1002/cbic.201800106},
journal = {ChemBioChem: a European journal of chemical biology},
pages = {1433--1443},
title = {Steroid-based amphiphiles for membrane protein study: the importance of alkyl spacers for protein stability},
url = {http://dx.doi.org/10.1002/cbic.201800106},
volume = {19},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Membrane proteins allow effective communication between cells and organelles and their external environments. Maintaining membrane protein stability in a nonnative environment is the major bottleneck to their structural study. Detergents are widely used to extract membrane proteins from the membrane and to keep the extracted protein in a stable state for downstream characterisation. In this study, three sets of steroidbased amphiphiles—glycodiosgenin analogues (GDNs) and steroidbased pentasaccharides either lacking a linker (SPSs) or containing a linker (SPSLs)—have been developed as new chemical tools for membrane protein research. These detergents were tested with three membrane proteins in order to characterise their ability to extract membrane proteins from the membrane and to stabilise membrane proteins longterm. Some of the detergents, particularly the SPSLs, displayed favourable behaviour with the tested membrane proteins. This result indicates the potential utility of these detergents as chemical tools for membrane protein structural study and a critical role of the simple alkyl spacer in determining detergent efficacy.
AU - Ehsan,M
AU - Das,M
AU - Stern,V
AU - Du,Y
AU - Mortensen,JS
AU - Hariharan,P
AU - Byrne,B
AU - Loland,CJ
AU - Kobilka,BK
AU - Guan,L
AU - Chae,PS
DO - 10.1002/cbic.201800106
EP - 1443
PY - 2018///
SN - 1439-4227
SP - 1433
TI - Steroid-based amphiphiles for membrane protein study: the importance of alkyl spacers for protein stability
T2 - ChemBioChem: a European journal of chemical biology
UR - http://dx.doi.org/10.1002/cbic.201800106
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000437267100014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/76818
VL - 19
ER -