Imperial College London

ProfessorBernadetteByrne

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Molecular Membrane Biology
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Byrne:1995,
author = {Byrne, B and Fowler, PA and Fraser, M and Culler, M and Templeton, A},
journal = {Biology of Reproduction},
pages = {88--95},
title = {GnSAF bioactivity present in serum from superovulated women is not blocked by inhibin antibody.},
volume = {52},
year = {1995}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Primary pituitary cultures from adult female rats were used to investigate gonadotropin surge attenuating factor (GnSAF) bioactivity. Serum from superovulated women was charcoal-treated and incubated with either an inhibin antibody (MC4), normal sheep serum (NSS), or serum-free defined medium (SFDM) before addition to cell culture. The reduction in GnRH-induced LH secretion (GnSAF bioactivity) produced by the serum (30.1 +/- 6.5%, p < 0.001, of control) was not altered by prior incubation with either MC4 or NSS (24.9 +/- 3.6 and 23.2 +/- 2.7%, p < 0.001, of control, respectively). This indicates that GnSAF bioactivity present in serum from superovulated women is not entirely attributable to inhibin. Recombinant human inhibin reduced basal FSH secretion to 24.6 +/- 4.7% (p < 0.001) of the control value. However, this was totally abolished by prior incubation with MC4, but not NSS. We have also shown that ovarian steroids are not responsible for GnSAF bioactivity in vitro. In conclusion, in contrast to findings in superovulated rats, inhibin antibody did not block GnSAF bioactivity in serum from superovulated women.
AU - Byrne,B
AU - Fowler,PA
AU - Fraser,M
AU - Culler,M
AU - Templeton,A
EP - 95
PY - 1995///
SP - 88
TI - GnSAF bioactivity present in serum from superovulated women is not blocked by inhibin antibody.
T2 - Biology of Reproduction
VL - 52
ER -