Imperial College London

ProfessorBernadetteByrne

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Molecular Membrane Biology
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Byrne:2015:10.1016/j.sbi.2015.01.005,
author = {Byrne, B},
doi = {10.1016/j.sbi.2015.01.005},
journal = {Current Opinion in Structural Biology},
pages = {9--17},
title = {Pichia pastoris as an expression host for membrane protein structural biology},
url = {http://dx.doi.org/10.1016/j.sbi.2015.01.005},
volume = {32},
year = {2015}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The methylotrophic yeast Pichia pastoris is a widely used recombinant expression host. P. pastoris combines the advantages of ease of use, relatively rapid expression times and low cost with eukaryotic co-translational and post-translational processing systems and lipid composition. The suitability of P. pastoris for high density controlled culture in bioreactors means large amounts of protein can be obtained from small culture volumes. This review details the key features of P. pastoris, which have made it a particularly useful system for the production of membrane proteins, including receptors, channels and transporters, for structural studies. In addition, this review provides an overview of all the constructs and cell strains used to produce membrane proteins, which have yielded high resolution structures.
AU - Byrne,B
DO - 10.1016/j.sbi.2015.01.005
EP - 17
PY - 2015///
SN - 0959-440X
SP - 9
TI - Pichia pastoris as an expression host for membrane protein structural biology
T2 - Current Opinion in Structural Biology
UR - http://dx.doi.org/10.1016/j.sbi.2015.01.005
UR - http://www.sciencedirect.com/science/article/pii/S0959440X15000068
UR - http://hdl.handle.net/10044/1/19203
VL - 32
ER -