Imperial College London

ProfessorBernadetteByrne

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Molecular Membrane Biology
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ehsan:2016:10.1021/jacs.5b13233,
author = {Ehsan, M and Du, Y and Scull, NJ and Tikhonova, E and Tarrasch, J and Mortensen, JS and Loland, CJ and Skiniotis, G and Guan, L and Byrne, B and Kobilka, BK and Chae, PS},
doi = {10.1021/jacs.5b13233},
journal = {Journal of the American Chemical Society},
pages = {3789--3796},
title = {Highly branched pentasaccharide-bearing amphiphiles for membrane protein studies},
url = {http://dx.doi.org/10.1021/jacs.5b13233},
volume = {138},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Detergents are essential tools for membrane protein manipulation. Micelles formed by detergent molecules have the ability to encapsulate the hydrophobic domains of membrane proteins. The resulting protein–detergent complexes (PDCs) are compatible with the polar environments of aqueous media, making structural and functional analysis feasible. Although a number of novel agents have been developed to overcome the limitations of conventional detergents, most have traditional head groups such as glucoside or maltoside. In this study, we introduce a class of amphiphiles, the PSA/Es with a novel highly branched pentasaccharide hydrophilic group. The PSA/Es conferred markedly increased stability to a diverse range of membrane proteins compared to conventional detergents, indicating a positive role for the new hydrophilic group in maintaining the native protein integrity. In addition, PDCs formed by PSA/Es were smaller and more suitable for electron microscopic analysis than those formed by DDM, indicating that the new agents have significant potential for the structure–function studies of membrane proteins.
AU - Ehsan,M
AU - Du,Y
AU - Scull,NJ
AU - Tikhonova,E
AU - Tarrasch,J
AU - Mortensen,JS
AU - Loland,CJ
AU - Skiniotis,G
AU - Guan,L
AU - Byrne,B
AU - Kobilka,BK
AU - Chae,PS
DO - 10.1021/jacs.5b13233
EP - 3796
PY - 2016///
SN - 1520-5126
SP - 3789
TI - Highly branched pentasaccharide-bearing amphiphiles for membrane protein studies
T2 - Journal of the American Chemical Society
UR - http://dx.doi.org/10.1021/jacs.5b13233
UR - https://pubs.acs.org/doi/10.1021/jacs.5b13233
UR - http://hdl.handle.net/10044/1/32292
VL - 138
ER -