Imperial College London

ProfessorBernadetteByrne

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Molecular Membrane Biology
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hussain:2016:10.1002/chem.201600533,
author = {Hussain, H and Du, Y and Scull, NJ and Mortensen, JS and Tarrasch, J and Bae, HE and Loland, CJ and Byrne, B and Kobilka, BK and Chae, PS},
doi = {10.1002/chem.201600533},
journal = {Chemistry},
title = {Accessible mannitol-based amphiphiles (MNAs) for membrane protein solubilisation and stabilisation},
url = {http://dx.doi.org/10.1002/chem.201600533},
volume = {22},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Integral membrane proteins are amphipathic molecules crucial for all cellular life. The structural study of these macromolecules starts with protein extraction from the native membranes, followed by purification and crystallisation. Detergents are essential tools for these processes, but detergent-solubilised membrane proteins often denature and aggregate, resulting in loss of both structure and function. In this study, a novel class of agents, designated mannitol-based amphiphiles (MNAs), were prepared and characterised for their ability to solubilise and stabilise membrane proteins. Some of MNAs conferred enhanced stability to four membrane proteins including a G protein-coupled receptor (GPCR), the β2 adrenergic receptor (β2AR), compared to both n-dodecyl-d-maltoside (DDM) and the other MNAs. These agents were also better than DDM for electron microscopy analysis of the β2AR. The ease of preparation together with the enhanced membrane protein stabilisation efficacy demonstrates the value of these agents for future membrane protein research.
AU - Hussain,H
AU - Du,Y
AU - Scull,NJ
AU - Mortensen,JS
AU - Tarrasch,J
AU - Bae,HE
AU - Loland,CJ
AU - Byrne,B
AU - Kobilka,BK
AU - Chae,PS
DO - 10.1002/chem.201600533
PY - 2016///
SN - 0861-9255
TI - Accessible mannitol-based amphiphiles (MNAs) for membrane protein solubilisation and stabilisation
T2 - Chemistry
UR - http://dx.doi.org/10.1002/chem.201600533
UR - http://hdl.handle.net/10044/1/32298
VL - 22
ER -