Imperial College London

ProfessorBernadetteByrne

Faculty of Natural SciencesDepartment of Life Sciences

Professor of Molecular Membrane Biology
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

101 results found

Ehsan M, Kumar A, Mortensen JS, Du Y, Hariharan P, Kumar KK, Ha B, Byrne B, Guan L, Kobilka BK, Loland CJ, Chae PSet al., 2019, Self-Assembly Behaviors of a Penta-Phenylene Maltoside and Its Application for Membrane Protein Study., Chem Asian J

We prepared an amphiphile with a penta-phenylene lipophilic group and a branched trimaltoside head group. This new agent, designated penta-phenylene maltoside (PPM), showed a marked tendency to self-assembly into micelles via strong aromatic-aromatic interactions in aqueous media, as evidenced by 1 H NMR spectroscopy and fluorescence studies. When utilized for membrane protein studies, this new agent was superior to DDM, a gold standard conventional detergent, in stabilizing multiple proteins long term. The ability of this agent to form aromatic-aromatic interactions is likely responsible for enhanced protein stabilization when associated with a target membrane protein.

JOURNAL ARTICLE

Das M, Du Y, Mortensen JS, Ramos M, Ghani L, Lee HJ, Bae HE, Byrne B, Guan L, Loland CJ, Kobilka BK, Chae PSet al., 2019, Trehalose-cored amphiphiles for membrane protein stabilization: importance of the detergent micelle size in GPCR stability., Org Biomol Chem, Vol: 17, Pages: 3249-3257

Despite their importance in biology and medicinal chemistry, structural and functional studies of membrane proteins present major challenges. To study diverse membrane proteins, it is crucial to have the correct detergent to efficiently extract and stabilize the proteins from the native membranes for biochemical/biophysical downstream analyses. But many membrane proteins, particularly eukaryotic ones, are recalcitrant to stabilization and/or crystallization with currently available detergents and thus there are major efforts to develop novel detergents with enhanced properties. Here, a novel class of trehalose-cored amphiphiles are introduced, with multiple alkyl chains and carbohydrates projecting from the trehalose core unit are introduced. A few members displayed enhanced protein stabilization behavior compared to the benchmark conventional detergent, n-dodecyl-β-d-maltoside (DDM), for multiple tested membrane proteins: (i) a bacterial leucine transporter (LeuT), (ii) the R. capsulatus photosynthetic superassembly, and (iii) the human β2 adrenergic receptor (β2AR). Due to synthetic convenience and their favourable behaviors for a range of membrane proteins, these agents have potential for membrane protein research. In addition, the detergent property-efficacy relationship discussed here will guide future design of novel detergents.

JOURNAL ARTICLE

Cecchetti C, Pyle E, Byrne B, 2019, Transporter oligomerisation: roles in structure and function, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 47, Pages: 433-440, ISSN: 0300-5127

JOURNAL ARTICLE

Hussain H, Helton T, Du Y, Mortensen JS, Hariharan P, Ehsan M, Byrne B, Loland CJ, Kobilka BK, Guan L, Chae PSet al., 2018, A comparative study of branched and linear mannitol-based amphiphiles on membrane protein stability, ANALYST, Vol: 143, Pages: 5702-5710, ISSN: 0003-2654

JOURNAL ARTICLE

Das M, Du Y, Mortensen JS, Hariharan P, Lee HS, Byrne B, Loland CJ, Guan L, Kobilka BK, Chae PSet al., 2018, Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy, CHEMBIOCHEM, Vol: 19, Pages: 2225-2232, ISSN: 1439-4227

JOURNAL ARTICLE

Pyle E, Kalli AC, Amillis S, Hall Z, Lau AM, Hanyaloglu AC, Diallinas G, Byrne B, Politis Aet al., 2018, Structural Lipids Enable the Formation of Functional Oligomers of the Eukaryotic Purine Symporter UapA, CELL CHEMICAL BIOLOGY, Vol: 25, Pages: 840-+, ISSN: 2451-9448

JOURNAL ARTICLE

Das M, Du Y, Mortensen JS, Bae HE, Byrne B, Loland CJ, Kobilka BK, Chae PSet al., 2018, An Engineered Lithocholate-Based Facial Amphiphile Stabilizes Membrane Proteins: Assessing the Impact of Detergent Customizability on Protein Stability, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 24, Pages: 9860-9868, ISSN: 0947-6539

JOURNAL ARTICLE

Ehsan M, Das M, Stern V, Du Y, Mortensen JS, Hariharan P, Byrne B, Loland CJ, Kobilka BK, Guan L, Chae PSet al., 2018, Steroid-Based Amphiphiles for Membrane Protein Study: The Importance of Alkyl Spacers for Protein Stability, CHEMBIOCHEM, Vol: 19, Pages: 1433-1443, ISSN: 1439-4227

JOURNAL ARTICLE

Ehsan M, Du Y, Molist I, Seven AB, Hariharan P, Mortensen JS, Ghani L, Loland CJ, Skiniotis G, Guan L, Byrne B, Kobilka BK, Chae PSet al., 2018, Vitamin E-based glycoside amphiphiles for membrane protein structural studies, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol: 16, Pages: 2489-2498, ISSN: 1477-0520

JOURNAL ARTICLE

Sadaf A, Du Y, Santillan C, Mortensen JS, Molist I, Seven AB, Hariharan P, Skiniotis G, Loland CJ, Kobilka BK, Guan L, Byrne B, Chae PSet al., 2017, Dendronic trimaltoside amphiphiles (DTMs) for membrane protein study, CHEMICAL SCIENCE, Vol: 8, Pages: 8315-8324, ISSN: 2041-6520

JOURNAL ARTICLE

Ehsan M, Ghani L, Du Y, Hariharan P, Mortensen JS, Ribeiro O, Hu H, Skiniotis G, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PSet al., 2017, New penta-saccharide-bearing tripod amphiphiles for membrane protein structure studies, ANALYST, Vol: 142, Pages: 3889-3898, ISSN: 0003-2654

JOURNAL ARTICLE

Pyle E, Kalli A, Amillis S, Hall Z, Hanyaloglu A, Diallinas G, Byrne B, Politis Aet al., 2017, Structural lipids enable the formation of functional oligomers of the eukaryotic purine symporter UapA

The role of membrane lipids in modulating eukaryotic transporter structure and function remains poorly understood. We used native mass spectrometry in combination with molecular dynamics simulations and in vivo analyses to investigate the roles of membrane lipids in the structure and transport activity of the purine transporter, UapA, from Aspergillus nidulans. We revealed that UapA exists mainly as a dimer and that two lipid molecules bind per UapA dimer. We identified three classes of phospholipids: phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) which co-purified with UapA. Delipidation of UapA caused dissociation of the dimer into individual protomers. Subsequent addition of PI or PE rescued the UapA dimer and allowed recovery of bound lipids, suggesting a central role of these lipids in stabilising the dimer. We predicted a putative lipid-binding site near the UapA dimer interface. Mutational analyses established that lipid binding at this site is essential for formation of functional UapA dimers. Our findings reveal unprecedented level of detail into the nature of UapA-lipid interactions and provide a framework for studying similar eukaryotic systems.

WORKING PAPER

Byrne B, 2017, It takes two to transport via an elevator, CELL RESEARCH, Vol: 27, Pages: 965-966, ISSN: 1001-0602

JOURNAL ARTICLE

Hussain H, Mortensen JS, Du Y, Santillan C, Ribeiro O, Go J, Hariharan P, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PSet al., 2017, Tandem malonate-based glucosides (TMGs) for membrane protein structural studies, SCIENTIFIC REPORTS, Vol: 7, ISSN: 2045-2322

JOURNAL ARTICLE

Hussain H, Du Y, Tikhonova E, Mortensen JS, Ribeiro O, Santillan C, Das M, Ehsan M, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PSet al., 2017, Resorcinarene-Based Facial Glycosides: Implication of Detergent Flexibility on Membrane-Protein Stability, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 23, Pages: 6724-6729, ISSN: 0947-6539

JOURNAL ARTICLE

Das M, Du Y, Ribeiro O, Hariharan P, Mortensen JS, Patra D, Skiniotis G, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PSet al., 2017, Conformationally Preorganized Diastereomeric Norbornane-Based Maltosides for Membrane Protein Study: Implications of Detergent Kink for Micellar Properties, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 139, Pages: 3072-3081, ISSN: 0002-7863

JOURNAL ARTICLE

Das M, Du Y, Mortensen JS, Ribeiro O, Hariharan P, Guan L, Loland CJ, Kobilka BK, Byrne B, Chae PSet al., 2017, Butane-1,2,3,4-tetraol-based amphiphilic stereoisomers for membrane protein study: importance of chirality in the linker region, CHEMICAL SCIENCE, Vol: 8, Pages: 1169-1177, ISSN: 2041-6520

JOURNAL ARTICLE

Cho KH, Ribeiro O, Du Y, Tikhonova E, Mortensen JS, Markham K, Hariharan P, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PSet al., 2016, Mesitylene-Cored Glucoside Amphiphiles (MGAs) for Membrane Protein Studies: Importance of Alkyl Chain Density in Detergent Efficacy, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 22, Pages: 18833-18839, ISSN: 0947-6539

JOURNAL ARTICLE

Alguel Y, Cameron AD, Diallinas G, Byrne Bet al., 2016, Transporter oligomerization: form and function, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 44, Pages: 1737-1744, ISSN: 0300-5127

JOURNAL ARTICLE

Cho KH, Hariharan P, Mortensen JS, Du Y, Nielsen AK, Byrne B, Kobilka BK, Loland CJ, Guan L, Chae PSet al., 2016, Isomeric Detergent Comparison for Membrane Protein Stability: Importance of Inter-Alkyl-Chain Distance and Alkyl Chain Length, CHEMBIOCHEM, Vol: 17, Pages: 2334-2339, ISSN: 1439-4227

JOURNAL ARTICLE

Boulet-Audet M, Kazarian SG, Byrne B, 2016, In-column ATR-FTIR spectroscopy to monitor affinity chromatography purification of monoclonal antibodies, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Carlsson E, Thwaite JE, Jenner DC, Spear AM, Flick-Smith H, Atkins HS, Byrne B, Ding JLet al., 2016, Bacillus anthracis TIR Domain-Containing Protein Localises to Cellular Microtubule Structures and Induces Autophagy, PLOS ONE, Vol: 11, ISSN: 1932-6203

JOURNAL ARTICLE

Hussain H, Du Y, Scull NJ, Mortensen JS, Tarrasch J, Bae HE, Loland CJ, Byrne B, Kobilka BK, Chae PSet al., 2016, Accessible Mannitol-Based Amphiphiles (MNAs) for Membrane Protein Solubilisation and Stabilisation, CHEMISTRY-A EUROPEAN JOURNAL, Vol: 22, Pages: 7068-7073, ISSN: 0947-6539

JOURNAL ARTICLE

Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD, Byrne Bet al., 2016, Structure of eukaryotic purine/H+ symporter UapA suggests a role for homodimerization in transport activity, NATURE COMMUNICATIONS, Vol: 7, ISSN: 2041-1723

JOURNAL ARTICLE

Ehsan M, Du Y, Scull NJ, Tikhonova E, Tarrasch J, Mortensen JS, Loland CJ, Skiniotis G, Guan L, Byrne B, Kobilka BK, Chae PSet al., 2016, Highly Branched Pentasaccharide-Bearing Amphiphiles for Membrane Protein Studies, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 138, Pages: 3789-3796, ISSN: 0002-7863

JOURNAL ARTICLE

Carlsson E, Ding JL, Byrne B, 2016, SARM modulates MyD88-mediated TLR activation through BB-loop dependent TIR-TIR interactions, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, Vol: 1863, Pages: 244-253, ISSN: 0167-4889

JOURNAL ARTICLE

Sadaf A, Mortensen JS, Capaldi S, Tikhonova E, Hariharan P, Ribeiro O, Loland CJ, Guan L, Byrne B, Chae PSet al., 2016, A class of rigid linker-bearing glucosides for membrane protein structural study, CHEMICAL SCIENCE, Vol: 7, Pages: 1933-1939, ISSN: 2041-6520

JOURNAL ARTICLE

Bae HE, Mortensen JS, Ribeiro O, Du Y, Ehsan M, Kobilka BK, Loland CJ, Byrne B, Chae PSet al., 2016, Tandem neopentyl glycol maltosides (TNMs) for membrane protein stabilisation, CHEMICAL COMMUNICATIONS, Vol: 52, Pages: 12104-12107, ISSN: 1359-7345

JOURNAL ARTICLE

Boulet-Audet M, Byrne B, Kazarian SG, 2015, Cleaning-in-place of immunoaffinity resins monitored by in situ ATR-FTIR spectroscopy, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol: 407, Pages: 7111-7122, ISSN: 1618-2642

JOURNAL ARTICLE

Martzoukou O, Karachaliou M, Yalelis V, Leung J, Byrne B, Amillis S, Diallinas Get al., 2015, Oligomerization of the UapA Purine Transporter Is Critical for ER-Exit, Plasma Membrane Localization and Turnover, JOURNAL OF MOLECULAR BIOLOGY, Vol: 427, Pages: 2679-2696, ISSN: 0022-2836

JOURNAL ARTICLE

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