Imperial College London
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ProfessorBernadetteByrne

Faculty of Natural Sciences, Department of Life Sciences

Associate Dean (Equality, Diversity and Inclusion) for FoNS
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Das:2018:10.1002/chem.201801141,
author = {Das, M and Du, Y and Mortensen, JS and Bae, HE and Byrne, B and Loland, CJ and Kobilka, BK and Chae, PS},
doi = {10.1002/chem.201801141},
journal = {Chemistry: A European Journal},
pages = {9860--9868},
title = {An engineered lithocholate-based facial amphiphile stabilizes membrane proteins: assessing the impact of detergent customizability on protein stability},
url = {http://dx.doi.org/10.1002/chem.201801141},
volume = {24},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Amphiphiles are critical tools for the structural and functional study of membrane proteins. Membrane proteins encapsulated by conventional headtotail detergents tend to undergo structural degradation, necessitating the development of structurally novel agents with improved efficacy. In recent years, facial amphiphiles have yielded encouraging results in terms of membrane protein stability. Herein, we report a new facial detergent (i.e., LFAC4) that confers greater stability to tested membrane proteins than the bola form analogue. Owing to the increased facial property and the adaptability of the detergent micelles in complex with different membrane proteins, LFAC4 yields increased stability compared to ndodecylβdmaltoside (DDM). Thus, this study not only describes a novel maltoside detergent with enhanced proteinstabilizing properties, but also shows that the customizable nature of a detergent plays an important role in the stabilization of membrane proteins. Owing to both synthetic convenience and enhanced stabilization efficacy for a range of membrane proteins, the new agent has major potential in membrane protein research.
AU  - Das,M
AU  - Du,Y
AU  - Mortensen,JS
AU  - Bae,HE
AU  - Byrne,B
AU  - Loland,CJ
AU  - Kobilka,BK
AU  - Chae,PS
DO  - 10.1002/chem.201801141
EP  - 9868
PY  - 2018///
SN  - 0947-6539
SP  - 9860
TI  - An engineered lithocholate-based facial amphiphile stabilizes membrane proteins: assessing the impact of detergent customizability on protein stability
T2  - Chemistry: A European Journal
UR  - http://dx.doi.org/10.1002/chem.201801141
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000438198000023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/76819
VL  - 24
ER  -
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