Imperial College London
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ProfessorBernadetteByrne

Faculty of Natural Sciences, Department of Life Sciences

Associate Dean (Equality, Diversity and Inclusion) for FoNS
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sadaf:2015:10.1039/c5sc02900g,
author = {Sadaf, A and Mortensen, JS and Capaldi, S and Tikhonova, E and Hariharan, P and Ribeiro, O and Loland, CJ and Guan, L and Byrne, B and Chae, PS},
doi = {10.1039/c5sc02900g},
journal = {Chemical Science},
pages = {1933--1939},
title = {A class of rigid linker-bearing glucosides for membrane protein structural study},
url = {http://dx.doi.org/10.1039/c5sc02900g},
volume = {7},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Membrane proteins are amphipathic bio-macromolecules incompatible with the polar environments ofaqueous media. Conventional detergents encapsulate the hydrophobic surfaces of membrane proteinsallowing them to exist in aqueous solution. Membrane proteins stabilized by detergent micelles are usedfor structural and functional analysis. Despite the availability of a large number of detergents, only a fewagents are sufficiently effective at maintaining the integrity of membrane proteins to allow successfulcrystallization. In the present study, we describe a novel class of synthetic amphiphiles with a branchedtail group and a triglucoside head group. These head and tail groups were connected via an amide orether linkage by using a tris(hydroxylmethyl)aminomethane (TRIS) or neopentyl glycol (NPG) linker toproduce TRIS-derived triglucosides (TDTs) and NPG-derived triglucosides (NDTs), respectively. Membersof this class conferred enhanced stability on target membrane proteins compared to conventionaldetergents. Because of straightforward synthesis of the novel agents and their favourable effects ona range of membrane proteins, these agents should be of wide applicability to membrane protein science.
AU  - Sadaf,A
AU  - Mortensen,JS
AU  - Capaldi,S
AU  - Tikhonova,E
AU  - Hariharan,P
AU  - Ribeiro,O
AU  - Loland,CJ
AU  - Guan,L
AU  - Byrne,B
AU  - Chae,PS
DO  - 10.1039/c5sc02900g
EP  - 1939
PY  - 2015///
SN  - 2041-6539
SP  - 1933
TI  - A class of rigid linker-bearing glucosides for membrane protein structural study
T2  - Chemical Science
UR  - http://dx.doi.org/10.1039/c5sc02900g
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000371021900037&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/31110
VL  - 7
ER  -
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