Imperial College London
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ProfessorBernadetteByrne

Faculty of Natural Sciences, Department of Life Sciences

Associate Dean (Equality, Diversity and Inclusion) for FoNS
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Carlsson:2016:10.1371/journal.pone.0158575,
author = {Carlsson, E and Thwaite, JE and Jenner, DC and Spear, AM and Flick-Smith, H and Atkins, HS and Byrne, B and Ding, JL},
doi = {10.1371/journal.pone.0158575},
journal = {PLOS One},
title = {Bacillus anthracis TIR Domain-Containing Protein Localises to Cellular Microtubule Structures and Induces Autophagy},
url = {http://dx.doi.org/10.1371/journal.pone.0158575},
volume = {11},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Toll-like receptors (TLRs) recognise invading pathogens and mediate downstream immune signalling via Toll/IL-1 receptor (TIR) domains. TIR domain proteins (Tdps) have been identified in multiple pathogenic bacteria and have recently been implicated as negative regulators of host innate immune activation. A Tdp has been identified in Bacillus anthracis, the causative agent of anthrax. Here we present the first study of this protein, designated BaTdp. Recombinantly expressed and purified BaTdp TIR domain interacted with several human TIR domains, including that of the key TLR adaptor MyD88, although BaTdp expression in cultured HEK293 cells had no effect on TLR4- or TLR2- mediated immune activation. During expression in mammalian cells, BaTdp localised to microtubular networks and caused an increase in lipidated cytosolic microtubule-associated protein 1A/1B-light chain 3 (LC3), indicative of autophagosome formation. In vivo intra-nasal infection experiments in mice showed that a BaTdp knockout strain colonised host tissue faster with higher bacterial load within 4 days post-infection compared to the wild type B. anthracis. Taken together, these findings indicate that BaTdp does not play an immune suppressive role, but rather, its absence increases virulence. BaTdp present in wild type B. anthracis plausibly interact with the infected host cell, which undergoes autophagy in self-defence.
AU  - Carlsson,E
AU  - Thwaite,JE
AU  - Jenner,DC
AU  - Spear,AM
AU  - Flick-Smith,H
AU  - Atkins,HS
AU  - Byrne,B
AU  - Ding,JL
DO  - 10.1371/journal.pone.0158575
PY  - 2016///
SN  - 1932-6203
TI  - Bacillus anthracis TIR Domain-Containing Protein Localises to Cellular Microtubule Structures and Induces Autophagy
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0158575
UR  - http://hdl.handle.net/10044/1/34085
VL  - 11
ER  -
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