Imperial College London
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ProfessorBernadetteByrne

Faculty of Natural Sciences, Department of Life Sciences

Associate Dean (Equality, Diversity and Inclusion) for FoNS
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cho:2016:10.1002/chem.201603338,
author = {Cho, KH and Ribeiro, O and Du, Y and Tikhonova, E and Mortensen, JS and Markham, K and Hariharan, P and Loland, CJ and Guan, L and Kobilka, BK and Byrne, B and Chae, PS},
doi = {10.1002/chem.201603338},
journal = {Chemistry - A European Journal},
title = {Mesitylene-cored glucoside amphiphiles (MGAs) for membrane protein studies: importance of alkyl chain density in detergent efficacy.},
url = {http://dx.doi.org/10.1002/chem.201603338},
volume = {22},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Detergents serve as useful tools for membrane protein structural and functional studies. Their amphipathic nature allows detergents to associate with the hydrophobic regions of membrane proteins whilst maintaining the proteins in aqueous solution. However, widely used conventional detergents are limited in their ability to maintain the structural integrity of membrane proteins and thus there are major efforts underway to develop novel agents with improved properties. We prepared mesitylene-cored glucoside amphiphiles (MGAs) with three alkyl chains and compared these agents with previously developed xylene-linked maltoside agents (XMAs) with two alkyl chains and a conventional detergent (DDM). When these agents were evaluated for four membrane proteins including a G protein-coupled receptor (GPCR), some agents such as MGA-C13 and MGA-C14 resulted in markedly enhanced stability of membrane proteins compared to both DDM and the XMAs. This favourable behaviour is due likely to the increased hydrophobic density provided by the extra alkyl chain. Thus, this study not only describes new glucoside agents with potential for membrane protein research, but also introduces a new detergent design principle for future development.
AU  - Cho,KH
AU  - Ribeiro,O
AU  - Du,Y
AU  - Tikhonova,E
AU  - Mortensen,JS
AU  - Markham,K
AU  - Hariharan,P
AU  - Loland,CJ
AU  - Guan,L
AU  - Kobilka,BK
AU  - Byrne,B
AU  - Chae,PS
DO  - 10.1002/chem.201603338
PY  - 2016///
SN  - 0947-6539
TI  - Mesitylene-cored glucoside amphiphiles (MGAs) for membrane protein studies: importance of alkyl chain density in detergent efficacy.
T2  - Chemistry - A European Journal
UR  - http://dx.doi.org/10.1002/chem.201603338
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27743406
UR  - http://hdl.handle.net/10044/1/42929
VL  - 22
ER  -
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