Imperial College London
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ProfessorBernadetteByrne

Faculty of Natural Sciences, Department of Life Sciences

Associate Dean (Equality, Diversity and Inclusion) for FoNS
 
 
 
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Contact

 

+44 (0)20 7594 3004b.byrne Website

 
 
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Location

 

504Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ehsan:2017:10.1039/c7an01168g,
author = {Ehsan, M and Ghani, L and Du, Y and Hariharan, P and Mortensen, JS and Ribeiro, O and Hu, H and Skiniotis, G and Loland, CJ and Guan, L and Kobilka, BK and Byrne, B and Chae, PS},
doi = {10.1039/c7an01168g},
journal = {The Analyst},
pages = {3889--3898},
title = {New penta-saccharide-bearing tripod amphiphiles for membrane protein structure studies},
url = {http://dx.doi.org/10.1039/c7an01168g},
volume = {142},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Integral membrane proteins either alone or as complexes carry out a range of key cellular functions. Detergents are indispensable tools in the isolation of membrane proteins from biological membranes for downstream studies. Although a large number of techniques and tools, including a wide variety of detergents, are available, purification and structural characterization of many membrane proteins remain challenging. In the current study, a new class of tripod amphiphiles bearing two different penta-saccharide head groups, designated TPSs, were developed and evaluated for their ability to extract and stabilize a range of diverse membrane proteins. Variations in the structures of the detergent head and tail groups allowed us to prepare three sets of the novel agents with distinctive structures. Some TPSs (TPS-A8 and TPS-E7) were efficient at extracting two proteins in a functional state while others (TPS-E8 and TPS-E10L) conferred marked stability to all membrane proteins (and membrane protein complexes) tested here compared to a conventional detergent. Use of TPS-E10L led to clear visualization of a receptor-Gs complex using electron microscopy, indicating profound potential in membrane protein research.
AU  - Ehsan,M
AU  - Ghani,L
AU  - Du,Y
AU  - Hariharan,P
AU  - Mortensen,JS
AU  - Ribeiro,O
AU  - Hu,H
AU  - Skiniotis,G
AU  - Loland,CJ
AU  - Guan,L
AU  - Kobilka,BK
AU  - Byrne,B
AU  - Chae,PS
DO  - 10.1039/c7an01168g
EP  - 3898
PY  - 2017///
SN  - 0003-2654
SP  - 3889
TI  - New penta-saccharide-bearing tripod amphiphiles for membrane protein structure studies
T2  - The Analyst
UR  - http://dx.doi.org/10.1039/c7an01168g
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000412565700012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/76817
VL  - 142
ER  -
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