Imperial College London
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Dr. Beth Holder

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Maternal and Fetal Health
 
 
 
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Contact

 

+44 (0)20 7594 1773b.holder Website

 
 
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Location

 

3 008Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Timms:2022:10.1002/mnfr.202200013,
author = {Timms, K and Holder, B and Day, A and Mclaughlin, J and Forbes, KA and Westwood, M},
doi = {10.1002/mnfr.202200013},
journal = {Molecular Nutrition and Food Research},
pages = {1--12},
title = {Watermelon-derived extracellular vesicles influence human ex vivo placental cell behavior by altering intestinal secretions},
url = {http://dx.doi.org/10.1002/mnfr.202200013},
volume = {66},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - ScopeDuring pregnancy, mother-to-fetus transfer of nutrients is mediated by the placenta; sub-optimal placental development and/or function results in fetal growth restriction (FGR), and the attendant risk of stillbirth, neurodevelopmental delay, and non-communicable diseases in adulthood. A maternal diet high in fruit and vegetables lowers the risk of FGR but the association cannot be explained fully by known macro- and micronutrients.Methods and resultsThis study investigates if dietary-derived extracellular vesicles (EVs) can regulate placental function. The study characterizes the microRNA and protein cargo of EVs isolated from watermelon, show they are actively internalized by human intestinal epithelial cells in vitro, use mass spectrometry to demonstrate that they alter the intestinal secretome and bioinformatic analyses to predict the likely affected pathways in cells/tissues distal to gut. Application of the watermelon EV-modified intestinal secretome to human placental trophoblast cells and ex vivo tissue explants affects the trophoblast proteome and key aspects of trophoblast behavior, including migration and syncytialization.ConclusionDietary-derived plant EVs can modify intestinal communication with distal tissues, including the placenta. Harnessing the beneficial properties of dietary-derived plant EVs and/or exploiting their potential as natural delivery agents may provide new ways to improve placental function and reduce rates of FGR.
AU  - Timms,K
AU  - Holder,B
AU  - Day,A
AU  - Mclaughlin,J
AU  - Forbes,KA
AU  - Westwood,M
DO  - 10.1002/mnfr.202200013
EP  - 12
PY  - 2022///
SN  - 1613-4125
SP  - 1
TI  - Watermelon-derived extracellular vesicles influence human ex vivo placental cell behavior by altering intestinal secretions
T2  - Molecular Nutrition and Food Research
UR  - http://dx.doi.org/10.1002/mnfr.202200013
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000842795300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202200013
UR  - http://hdl.handle.net/10044/1/99704
VL  - 66
ER  -
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