Imperial College London
Portrait Picture

Dr. Beth Holder

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer in Maternal and Fetal Health
 
 
 
//

Contact

 

+44 (0)20 7594 1773b.holder Website

 
 
//

Location

 

3 008Institute of Reproductive and Developmental BiologyHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Holder:2016:10.1111/tra.12352,
author = {Holder, BS and Jones, T and Sancho, Shimizu V and Rice, TF and Donaldson, B and Bouqueau, M and Forbes, K and Kampmann, B},
doi = {10.1111/tra.12352},
journal = {Traffic},
pages = {168--178},
title = {Macrophage exosomes induce placental inflammatory cytokines: a novel mode of maternal-placental messaging},
url = {http://dx.doi.org/10.1111/tra.12352},
volume = {17},
year = {2016}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - During pregnancy, the placenta forms the interface between mother and fetus. Highly controlled regulation of trans-placental trafficking is therefore essential for the healthy development of the growing fetus. Extracellular vesicle-mediated transfer of protein and nucleic acids from the human placenta into the maternal circulation is well documented; the possibility that this trafficking is bi-directional has not yet been explored but could affect placental function and impact on the fetus. We hypothesized that the ability of the placenta to respond to maternal inflammatory signals is mediated by the interaction of maternal immune cell exosomes with placental trophoblast. Utilizing the BeWo cell line and whole placental explants, we demonstrated that the human placenta internalizes macrophage-derived exosomes in a time- and dose-dependent manner. This uptake was via clathrin-dependent endocytosis. Furthermore, macrophage exosomes induced release of proinflammatory cytokines by the placenta. Taken together, our data demonstrates that exosomes are actively transported into the human placenta and that exosomes from activated immune cells modulate placental cytokine production. This represents a novel mechanism by which immune cells can signal to the placental unit, potentially facilitating responses to maternal inflammation and infection, and thereby preventing harm to the fetus.
AU  - Holder,BS
AU  - Jones,T
AU  - Sancho,Shimizu V
AU  - Rice,TF
AU  - Donaldson,B
AU  - Bouqueau,M
AU  - Forbes,K
AU  - Kampmann,B
DO  - 10.1111/tra.12352
EP  - 178
PY  - 2016///
SN  - 1600-0854
SP  - 168
TI  - Macrophage exosomes induce placental inflammatory cytokines: a novel mode of maternal-placental messaging
T2  - Traffic
UR  - http://dx.doi.org/10.1111/tra.12352
UR  - http://hdl.handle.net/10044/1/28022
VL  - 17
ER  -
Download