Publications

BibTex format

@article{Juskaite:2019:10.21769/BioProtoc.3339,
author = {Juskaite, V and Leitinger, B},
doi = {10.21769/BioProtoc.3339},
journal = {Bio-protocol},
title = {Cell-based assay for recruitment of DDR1 to collagen-coated beads},
url = {http://dx.doi.org/10.21769/BioProtoc.3339},
volume = {9},
year = {2019}
}

Download

RIS format (EndNote, RefMan)

TY - JOUR
AB - The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for theextracellular matrix protein collagen. The interactions of cells with collagen are difficult to study becauseof the difficulty to obtain native collagen fibers for in vitro studies. Thus, in vitro studies often use acidsoluble collagens in the form of single triple helices, which are not representative of the densely packedinsoluble collagen fibers found in tissues. In this protocol, we describe a method that allows stimulatingDDR1 locally with collagen-coated beads. Latex beads are first coated with acid-soluble collagen, thenadded to cells expressing DDR1. Recruitment of DDR1 to the beads and collagen-induced DDR1phosphorylation is visualized by immunofluorescence microscopy on a widefield microscope. In thismethod, densely packed collagen is presented to cells in an insoluble form. Bead coating is easy toperform, and this method thus presents a straightforward protocol with which to study local recruitmentof collagen receptors to insoluble collagen.
AU - Juskaite,V
AU - Leitinger,B
DO - 10.21769/BioProtoc.3339
PY - 2019///
SN - 2331-8325
TI - Cell-based assay for recruitment of DDR1 to collagen-coated beads
T2 - Bio-protocol
UR - http://dx.doi.org/10.21769/BioProtoc.3339
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000482206700010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - http://hdl.handle.net/10044/1/73446
VL - 9
ER -

Download

DrBirgitLeitinger

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)