Imperial College London
Alternate

DrBirgitLeitinger

Faculty of Medicine, National Heart & Lung Institute

Reader in Matrix Receptor Signalling
 
 
 
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Contact

 

+44 (0)20 7594 1591b.leitinger Website

 
 
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Location

 

115Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Woltersdorf:2017:10.1016/j.matbio.2017.02.001,
author = {Woltersdorf, C and Bonk, M and Leitinger, B and Huhtala, M and Käpylä, J and Heino, J and Gil, Girol C and Niland, S and Eble, JA and Bruckner, P and Dreier, R and Hansen, U},
doi = {10.1016/j.matbio.2017.02.001},
journal = {Matrix Biology},
pages = {91--105},
title = {The binding capacity of α1β1-, α2β1- and α10β1-integrins depends on non-collagenous surface macromolecules rather than the collagens in cartilage fibrils.},
url = {http://dx.doi.org/10.1016/j.matbio.2017.02.001},
volume = {63},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Interactions of cells with supramolecular aggregates of the extracellular matrix (ECM) are mediated, in part, by cell surface receptors of the integrin family. These are important molecular components of cell surface-suprastructures regulating cellular activities in general. A subfamily of β1-integrins with von Willebrand-factor A-like domains (I-domains) in their α-chains can bind to collagen molecules and, therefore, are considered as important cellular mechano-receptors. Here we show that chondrocytes strongly bind to cartilage collagens in the form of individual triple helical molecules but very weakly to fibrils formed by the same molecules. We also find that chondrocyte integrins α1β1-, α2β1- and α10β1-integrins and their I-domains have the same characteristics. Nevertheless we find integrin binding to mechanically generated cartilage fibril fragments, which also comprise peripheral non-collagenous material. We conclude that cell adhesion results from binding of integrin-containing adhesion suprastructures to the non-collagenous fibril periphery but not to the collagenous fibril cores. The biological importance of the well-investigated recognition of collagen molecules by integrins is unknown. Possible scenarios may include fibrillogenesis, fibril degradation and/or phagocytosis, recruitment of cells to remodeling sites, or molecular signaling across cytoplasmic membranes. In these circumstances, collagen molecules may lack a fibrillar organization. However, other processes requiring robust biomechanical functions, such as fibril organization in tissues, cell division, adhesion, or migration, do not involve direct integrin-collagen interactions.
AU  - Woltersdorf,C
AU  - Bonk,M
AU  - Leitinger,B
AU  - Huhtala,M
AU  - Käpylä,J
AU  - Heino,J
AU  - Gil,Girol C
AU  - Niland,S
AU  - Eble,JA
AU  - Bruckner,P
AU  - Dreier,R
AU  - Hansen,U
DO  - 10.1016/j.matbio.2017.02.001
EP  - 105
PY  - 2017///
SN  - 1569-1802
SP  - 91
TI  - The binding capacity of α1β1-, α2β1- and α10β1-integrins depends on non-collagenous surface macromolecules rather than the collagens in cartilage fibrils.
T2  - Matrix Biology
UR  - http://dx.doi.org/10.1016/j.matbio.2017.02.001
UR  - http://hdl.handle.net/10044/1/45611
VL  - 63
ER  -
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