Imperial College London
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ProfessorBorisLenhard

Faculty of Medicine, Institute of Clinical Sciences

Professor of Computational Biology
 
 
 
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Contact

 

+44 (0)20 7594 0911b.lenhard Website

 
 
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Assistant

 

Mr Alastair Douglas Ivor Williams +44 (0)20 3313 4318

 
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Location

 

6.12CLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Yu:2020:10.1101/2020.06.08.118984,
author = {Yu, C and Cvetesic, N and Hisler, V and Gupta, K and Ye, T and Gazdag, E and Negroni, L and Hajkova, P and Berger, I and Lenhard, B and Müller, F and Vincent, SD and Tora, L},
doi = {10.1101/2020.06.08.118984},
title = {TBPL2/TFIIA complex establishes the maternal transcriptome by an oocyte-specific promoter usage},
url = {http://dx.doi.org/10.1101/2020.06.08.118984},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>During oocyte growth, transcription is required to create RNA and protein reserves to achieve maternal competence. During this period, the general transcription factor TATA binding protein (TBP) is replaced by its paralogue, TBPL2 (TBP2 or TRF3), which is essential for RNA polymerase II transcription. We show that in oocytes TBPL2 does not assemble into a canonical TFIID complex. Our transcript analyses demonstrate that TBPL2 mediates transcription of oocyte-expressed genes, including mRNA survey genes, as well as specific endogenous retroviral elements. Transcription start site (TSS) mapping indicates that TBPL2 has a strong preference for TATA-like motif in core promoters driving sharp TSS selection, in contrast with canonical TBP/TFIID-driven TATA-less promoters that have broader TSS architecture. Thus, we show a role for the TBPL2/TFIIA complex in the establishment of the oocyte transcriptome by using a specific TSS recognition code.</jats:p>
AU  - Yu,C
AU  - Cvetesic,N
AU  - Hisler,V
AU  - Gupta,K
AU  - Ye,T
AU  - Gazdag,E
AU  - Negroni,L
AU  - Hajkova,P
AU  - Berger,I
AU  - Lenhard,B
AU  - Müller,F
AU  - Vincent,SD
AU  - Tora,L
DO  - 10.1101/2020.06.08.118984
PY  - 2020///
TI  - TBPL2/TFIIA complex establishes the maternal transcriptome by an oocyte-specific promoter usage
UR  - http://dx.doi.org/10.1101/2020.06.08.118984
ER  -
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