Imperial College London
Portrait Picture

ProfessorBorisLenhard

Faculty of Medicine, Institute of Clinical Sciences

Professor of Computational Biology
 
 
 
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Contact

 

+44 (0)20 7594 0911b.lenhard Website

 
 
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Assistant

 

Mr Alastair Douglas Ivor Williams +44 (0)20 3313 4318

 
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Location

 

6.12CLMS BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Guo:2022:10.1016/j.molcel.2022.09.003,
author = {Guo, Y and Al-Jibury, E and Garcia-Millan, R and Ntagiantas, K and King, JWD and Nash, AJ and Galjart, N and Lenhard, B and Rueckert, D and Fisher, AG and Pruessner, G and Merkenschlager, M},
doi = {10.1016/j.molcel.2022.09.003},
journal = {Molecular Cell},
pages = {3769--3780.e5},
title = {Chromatin jets define the properties of cohesin-driven in vivo loop extrusion},
url = {http://dx.doi.org/10.1016/j.molcel.2022.09.003},
volume = {82},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Complex genomes show intricate organization in three-dimensional (3D) nuclear space. Current models posit that cohesin extrudes loops to form self-interacting domains delimited by the DNA binding protein CTCF. Here, we describe and quantitatively characterize cohesin-propelled, jet-like chromatin contacts as landmarks of loop extrusion in quiescent mammalian lymphocytes. Experimental observations and polymer simulations indicate that narrow origins of loop extrusion favor jet formation. Unless constrained by CTCF, jets propagate symmetrically for 1-2 Mb, providing an estimate for the range of in vivo loop extrusion. Asymmetric CTCF binding deflects the angle of jet propagation as experimental evidence that cohesin-mediated loop extrusion can switch from bi- to unidirectional and is controlled independently in both directions. These data offer new insights into the physiological behavior of in vivo cohesin-mediated loop extrusion and further our understanding of the principles that underlie genome organization.
AU  - Guo,Y
AU  - Al-Jibury,E
AU  - Garcia-Millan,R
AU  - Ntagiantas,K
AU  - King,JWD
AU  - Nash,AJ
AU  - Galjart,N
AU  - Lenhard,B
AU  - Rueckert,D
AU  - Fisher,AG
AU  - Pruessner,G
AU  - Merkenschlager,M
DO  - 10.1016/j.molcel.2022.09.003
EP  - 3780
PY  - 2022///
SN  - 1097-2765
SP  - 3769
TI  - Chromatin jets define the properties of cohesin-driven in vivo loop extrusion
T2  - Molecular Cell
UR  - http://dx.doi.org/10.1016/j.molcel.2022.09.003
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36182691
UR  - https://www.sciencedirect.com/science/article/pii/S1097276522008541?via%3Dihub
UR  - http://hdl.handle.net/10044/1/99990
VL  - 82
ER  -
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