Imperial College London
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Dr Benjamin Mullish

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

IPPRF Research Fellow
 
 
 
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Contact

 

b.mullish

 
 
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Location

 

Queen Elizabeth the Queen Mother Wing (QEQM)St Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Monaghan:2021:10.3390/cells10113234,
author = {Monaghan, TM and Duggal, NA and Rosati, E and Griffin, R and Hughes, J and Roach, B and Yang, DY and Wang, C and Wong, K and Saxinger, L and Pui-Bakovi, M and Vukovi, F and Klicek, F and Lauc, G and Tighe, P and Mullish, BH and Miguens, Blanco J and McDonald, JAK and Marchesi, JR and Xue, N and Dottorini, T and Acharjee, A and Franke, A and Wong, GK-S and Polytarchou, C and Yau, TO and Christodoulou, N and Hatziapostoulou, M and Wang, M and Russell, LA and Kao, DH},
doi = {10.3390/cells10113234},
journal = {Cells},
title = {A multi-factorial observational study on sequential fecal  microbiota transplant in patients with medically refractory Clostridioides difficile infection},
url = {http://dx.doi.org/10.3390/cells10113234},
volume = {10},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Fecal microbiota transplantation (FMT) is highly effective in recurrent Clostridioides difficile infection (CDI); increasing evidence supports FMT in severe or fulminant Clostridioides difficile infection (SFCDI). However, the multifactorial mechanisms that underpin the efficacy of FMT are not fully understood. Systems biology approaches using high-throughput technologies may help with mechanistic dissection of host-microbial interactions. Here, we have undertaken a deep phenomics study on four adults receiving sequential FMT for SFCDI, in which we performed a longitudinal, integrative analysis of multiple host factors and intestinal microbiome changes. Stool samples were profiled for changes in gut microbiota and metabolites and blood samples for alterations in targeted epigenomic, metabonomic, glycomic, immune proteomic, immunophenotyping, immune functional assays, and T-cell receptor (TCR) repertoires, respectively. We characterised temporal trajectories in gut microbial and host immunometabolic data sets in three responders and one non-responder to sequential FMT. A total of 562 features were used for analysis, of which 78 features were identified, which differed between the responders and the non-responder. The observed dynamic phenotypic changes may potentially suggest immunosenescent signals in the non-responder and may help to underpin the mechanisms accompanying successful FMT, although our study is limited by a small sample size and significant heterogeneity in patient baseline characteristics. Our multi-omics integrative longitudinal analytical approach extends the knowledge regarding mechanisms of efficacy of FMT and highlights preliminary novel signatures, which should be validated in larger studies.
AU  - Monaghan,TM
AU  - Duggal,NA
AU  - Rosati,E
AU  - Griffin,R
AU  - Hughes,J
AU  - Roach,B
AU  - Yang,DY
AU  - Wang,C
AU  - Wong,K
AU  - Saxinger,L
AU  - Pui-Bakovi,M
AU  - Vukovi,F
AU  - Klicek,F
AU  - Lauc,G
AU  - Tighe,P
AU  - Mullish,BH
AU  - Miguens,Blanco J
AU  - McDonald,JAK
AU  - Marchesi,JR
AU  - Xue,N
AU  - Dottorini,T
AU  - Acharjee,A
AU  - Franke,A
AU  - Wong,GK-S
AU  - Polytarchou,C
AU  - Yau,TO
AU  - Christodoulou,N
AU  - Hatziapostoulou,M
AU  - Wang,M
AU  - Russell,LA
AU  - Kao,DH
DO  - 10.3390/cells10113234
PY  - 2021///
SN  - 2073-4409
TI  - A multi-factorial observational study on sequential fecal  microbiota transplant in patients with medically refractory Clostridioides difficile infection
T2  - Cells
UR  - http://dx.doi.org/10.3390/cells10113234
UR  - https://www.mdpi.com/2073-4409/10/11/3234/htm
UR  - http://hdl.handle.net/10044/1/93046
VL  - 10
ER  -
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