Publications
169 results found
Liu Y, Feizi T, Campanero-Rhodes MA, et al., 2007, Neoglycolipid probes prepared via oxime ligation for microarray analysis of oligosaccharide-protein interactions, Chem.Biol., Vol: 14, Pages: 847-859
Neoglycolipid technology is the basis of a microarray platform for assigning oligosaccharide ligands for carbohydrate-binding proteins. The strategy for generating the neoglycolipid probes by reductive amination results in ring opening of the core monosaccharides. This often limits applicability to short-chain saccharides, although the majority of recognition motifs are satisfactorily presented with neoglycolipids of longer oligosaccharides. Here, we describe neoglycolipids prepared by oxime ligation. We provide evidence from NMR studies that a significant proportion of the oxime-linked core monosaccharide is in the ring-closed form, and this form selectively interacts with a carbohydrate-binding protein. By microarray analyses we demonstrate the effective presentation with oxime-linked neoglycolipids of (1) Lewis(x) trisaccharide to antibodies to Lewis(x), (2) sialyllactose analogs to the sialic acid-binding receptors, siglecs, and (3) N-glycans to a plant lectin that requires an intact N-acetylglucosamine core
Alfano I, Vora P, Mummery RS, et al., 2007, The major determinant of the heparin binding of glial cell-line-derived neurotrophic factor is near the N-terminus and is dispensable for receptor binding, BIOCHEMICAL JOURNAL, Vol: 404, Pages: 131-140, ISSN: 0264-6021
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- Citations: 27
Clark SJ, Higman VA, Mulloy B, et al., 2007, Characterisation of the CCP7 heparin-binding site in human complement factor H: Functional variation in AMD-associated allelic variant, 21st International Complement Workshop, Publisher: PERGAMON-ELSEVIER SCIENCE LTD, Pages: 161-161, ISSN: 0161-5890
Nermut MV, Mulloy B, 2007, Consideration of the three-dimensional structure of core shells (capsids) in spherical retroviruses, MICRON, Vol: 38, Pages: 462-470, ISSN: 0968-4328
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- Citations: 3
Sibille N, Sillen A, Leroy A, et al., 2006, Structural impact of heparin binding to full-length Tau as studied by NMR spectroscopy, BIOCHEMISTRY, Vol: 45, Pages: 12560-12572, ISSN: 0006-2960
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- Citations: 124
Clark SJ, Higman VA, Mulloy B, et al., 2006, His-384 allotypic variant of factor H associated with age-related macular degeneration has different heparin binding properties from the non-disease-associated form., JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 24713-24720
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- Citations: 150
Bittencourt VCB, Figueiredo RT, da Silva RB, et al., 2006, An α-glucan of <i>Pseudallescheria boydii</i> is involved in fungal phagocytosis and toll-like receptor activation, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 22614-22623
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- Citations: 113
Bittencourt VCB, Figueiredo RT, da Silva RB, et al., 2006, An alpha-glucan of Pseudallescheria boydii is involved in fungal phagocytosis and Toll-like receptor activation., J Biol Chem, Vol: 281, Pages: 22614-22623, ISSN: 0021-9258
The host response to fungi is in part dependent on activation of evolutionarily conserved receptors, including toll-like receptors and phagocytic receptors. However, the molecular nature of fungal ligands responsible for this activation is largely unknown. Herein, we describe the isolation and structural characterization of an alpha-glucan from Pseudallescheria boydii cell wall and evaluate its role in the induction of innate immune response. These analyses indicate that alpha-glucan of P. boydii is a glycogen-like polysaccharide consisting of linear 4-linked alpha-D-Glcp residues substituted at position 6 with alpha-D-Glcp branches. Soluble alpha-glucan, but not beta-glucan, led to a dose-dependent inhibition of conidia phagocytosis. Furthermore, a significant decrease in the phagocytic index occurred when alpha-glucan from conidial surface was removed by enzymatic treatment with alpha-amyloglucosidase, thus indicating an essential role of alpha-glucan in P. boydii internalization by macrophages. alpha-Glucan stimulates the secretion of inflammatory cytokines by macrophages and dendritic cells; again this effect is abolished by treatment with alpha-amyloglucosidase. Finally, alpha-glucan induces cytokine secretion by cells of the innate immune system in a mechanism involving toll-like receptor 2, CD14, and MyD88. These results might have relevance in the context of infections with P. boydii and other fungi, and alpha-glucan could be a target for intervention during fungal infections.
Kemp LE, Mulloy B, Gherardi E, 2006, Signalling by HGF/SF and Met: the role of heparan sulphate co-receptors, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 34, Pages: 414-417, ISSN: 0300-5127
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- Citations: 44
Forster M, Mulloy B, 2006, Computational approaches to the identification of heparin-binding sites on the surfaces of proteins, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 34, Pages: 431-434, ISSN: 0300-5127
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- Citations: 45
Mulloy B, Rider CC, 2006, Cytokines and proteoglycans: an introductory overview, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 34, Pages: 409-413, ISSN: 0300-5127
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- Citations: 59
Chai W, Piskarev VE, Mulloy B, et al., 2006, Analysis of chain and blood group type and branching pattern of sialylated oligosaccharides by negative ion electrospray tandem mass spectrometry, Anal.Chem., Vol: 78, Pages: 1581-1592
We previously reported sequence determination of neutral oligosaccharides by negative ion electrospray tandem mass spectrometry on a quadrupole-orthogonal time-of-flight instrument with high sensitivity and without the need of derivatization. In the present report, we extend our strategies to sialylated oligosaccharides for analysis of chain and blood group types together with branching patterns. A main feature in the negative ion mass spectrometry approach is the unique double glycosidic cleavage induced by 3-glycosidic substitution, producing characteristic D-type fragments which can be used to distinguish the type 1 and type 2 chains, the blood group related Lewis determinants, 3,6-disubstituted core branching patterns, and to assign the structural details of each of the branches. Twenty mono- and disialylated linear and branched oligosaccharides were used for the investigation, and the sensitivity achieved is in the femtomole range. To demonstrate the efficacy of the strategy, we have determined a novel complex disialylated and monofucosylated tridecasaccharide that is based on the lacto-N-decaose core. The structure and sequence assignment was corroborated by methylation analysis and 1H NMR spectroscopy
Robinson CJ, Mulloy B, Gallagher JT, et al., 2006, VEGF<sub>165</sub>-binding sites within heparan sulfate encompass two highly sulfated domains and can be liberated by K5 lyase, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 1731-1740
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- Citations: 137
Mulloy B, Rider CC, 2006, Cytokine/proteoglycan interactions: A biochemical society focused meeting (Royal Holloway University of London), Pages: 37-38, ISSN: 0954-982X
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- Citations: 1
Clark SJ, Higman VA, Fernando A, et al., 2006, Characterisation of a heparin binding domain in complement factor H, 10th Meeting on Complement in Human Disease, Publisher: PERGAMON-ELSEVIER SCIENCE LTD, Pages: 142-143, ISSN: 0161-5890
Mulloy B, 2006, Progress in the Structural Biology of Chondroitin Sulfate, CHONDROITIN SULFATE: STRUCTURE, ROLE AND PHARMACOLOGICAL ACTIVITY, Vol: 53, Pages: 49-67, ISSN: 1054-3589
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- Citations: 3
Mulloy B, 2005, The specificity of interactions between proteins and sulfated polysaccharides, ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, Vol: 77, Pages: 651-664, ISSN: 0001-3765
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- Citations: 89
Feizi T, Mulloy B, 2005, Carbohydrates and glycoconjugates: Never a dull moment in glycoscience, Current Opinion in Structural Biology, Vol: 15, Pages: 479-480, ISSN: 0959-440X
Feizi T, Mulloy B, 2005, Carbohydrates and glycoconjugates: never a dull moment in glycoscience - Editorial overview, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 15, Pages: 479-480, ISSN: 0959-440X
Pérez S, Mulloy B, 2005, Prospects for glycoinformatics, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 15, Pages: 517-524, ISSN: 0959-440X
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- Citations: 38
Pinto MR, Gorin PAJ, Wait R, et al., 2005, Structures of the <i>O</i>-linked oligosaccharides of a complex glycoconjugate from <i>Pseudallescheria boydii</i>, GLYCOBIOLOGY, Vol: 15, Pages: 895-904, ISSN: 0959-6658
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- Citations: 25
Mahoney DJ, Mulloy B, Forster MJ, et al., 2005, Characterization of the interaction between tumor necrosis factor-stimulated gene-6 and heparin - Implications for the inhibition of plasmin in extracellular matrix microenvironments, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 280, Pages: 27044-27055, ISSN: 0021-9258
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- Citations: 77
Griffitts JS, Haslam SM, Yang TL, et al., 2005, Glycolipids as receptors for <i>Bacillus thuringiensis</i> crystal toxin, SCIENCE, Vol: 307, Pages: 922-925, ISSN: 0036-8075
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- Citations: 269
Bavington CD, Lever R, Mulloy B, et al., 2004, Anti-adhesive glycoproteins in echinoderm mucus secretions, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, Vol: 139, Pages: 607-617, ISSN: 1096-4959
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- Citations: 45
Killeen R, Wait R, Begum S, et al., 2004, Identification of major heparin-binding proteins in plasma using electrophoresis and mass spectrometry, Meeting of the British-Society-for-Matrix-Biology, Publisher: BLACKWELL PUBLISHING LTD, Pages: A69-A69, ISSN: 0959-9673
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- Citations: 5
Mahoney DJ, Whittle JD, Milner CM, et al., 2004, A method for the non-covalent immobilization of heparin to surfaces, ANALYTICAL BIOCHEMISTRY, Vol: 330, Pages: 123-129, ISSN: 0003-2697
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- Citations: 46
Harmer NJ, Ilag LL, Mulloy B, et al., 2004, Towards a resolution of the stoichiometry of the fibroblast growth factor (FGF)-FGIF receptor - Heparin complex, JOURNAL OF MOLECULAR BIOLOGY, Vol: 339, Pages: 821-834, ISSN: 0022-2836
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- Citations: 87
Kariya Y, Mulloy B, Imai K, et al., 2004, Isolation and partial characterization of fucan sulfates from the body wall of sea cucumber Stichopus japonicus and their ability to inhibit osteoclastogenesis, CARBOHYDRATE RESEARCH, Vol: 339, Pages: 1339-1346, ISSN: 0008-6215
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- Citations: 103
Pinto MR, Mulloy B, Wait R, et al., 2003, Structures of the <i>O</i>-linked oligosaccharides of a complex glycoconjugate from <i>Pseudallescheria boydii</i>, 8th Annual Conference of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 852-852, ISSN: 0959-6658
Feizi T, Mulloy B, 2003, Carbohydrates and glycoconjugates glycomics: the new era of carbohydrate biology, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 13, Pages: 602-604, ISSN: 0959-440X
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- Citations: 35
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