Imperial College London
Alternate

DrBrianRobertson

Faculty of Medicine, Department of Infectious Disease

Reader in Systems Microbiology
 
 
 
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Contact

 

b.robertson

 
 
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Location

 

3.41Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Shahrezaei:2017:10.3389/fcell.2017.00064,
author = {Shahrezaei, V and Robertson, B and Thomas, P and Priestman, M},
doi = {10.3389/fcell.2017.00064},
journal = {Frontiers in Cell and Developmental Biology},
title = {Mycobacteria modify their cell size control under sub-optimal carbon sources},
url = {http://dx.doi.org/10.3389/fcell.2017.00064},
volume = {5},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The decision to divide is the most important one that any cell must make. Recent single cell studies suggest that most bacteria follow an “adder” model of cell size control, incorporating a fixed amount of cell wall material before dividing. Mycobacteria, including the causative agent of tuberculosis Mycobacterium tuberculosis, are known to divide asymmetrically resulting in heterogeneity in growth rate, doubling time, and other growth characteristics in daughter cells. The interplay between asymmetric cell division and adder size control has not been extensively investigated. Moreover, the impact of changes in the environment on growth rate and cell size control have not been addressed for mycobacteria. Here, we utilize time-lapse microscopy coupled with microfluidics to track live Mycobacterium smegmatis cells as they grow and divide over multiple generations, under a variety of growth conditions. We demonstrate that, under optimal conditions, M. smegmatis cells robustly follow the adder principle, with constant added length per generation independent of birth size, growth rate, and inherited pole age. However, the nature of the carbon source induces deviations from the adder model in a manner that is dependent on pole age. Understanding how mycobacteria maintain cell size homoeostasis may provide crucial targets for the development of drugs for the treatment of tuberculosis, which remains a leading cause of global mortality.
AU  - Shahrezaei,V
AU  - Robertson,B
AU  - Thomas,P
AU  - Priestman,M
DO  - 10.3389/fcell.2017.00064
PY  - 2017///
SN  - 2296-634X
TI  - Mycobacteria modify their cell size control under sub-optimal carbon sources
T2  - Frontiers in Cell and Developmental Biology
UR  - http://dx.doi.org/10.3389/fcell.2017.00064
UR  - http://hdl.handle.net/10044/1/49666
VL  - 5
ER  -
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