Imperial College London
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DrBenjaminSchumann

Faculty of Natural Sciences, Department of Chemistry

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 3796 5047b.schumann Website CV

 
 
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Location

 

Francis Crick InstituteThe Francis Crick Institute

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Summary

 

Publications

Citation

BibTex format

@article{Cioce:2021:10.1016/j.cbpa.2020.09.001,
author = {Cioce, A and Malaker, SA and Schumann, B},
doi = {10.1016/j.cbpa.2020.09.001},
journal = {Current Opinion in Chemical Biology},
pages = {66--78},
title = {Generating orthogonal glycosyltransferase and nucleotide sugar pairs as next-generation glycobiology tools},
url = {http://dx.doi.org/10.1016/j.cbpa.2020.09.001},
volume = {60},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Protein glycosylation fundamentally impacts biological processes. Nontemplated biosynthesis introduces unparalleled complexity into glycans that needs tools to understand their roles in physiology. The era of quantitative biology is a great opportunity to unravel these roles, especially by mass spectrometry glycoproteomics. However, with high sensitivity come stringent requirements on tool specificity. Bioorthogonal metabolic labeling reagents have been fundamental to studying the cell surface glycoproteome but typically enter a range of different glycans and are thus of limited specificity. Here, we discuss the generation of metabolic 'precision tools' to study particular subtypes of the glycome. A chemical biology tactic termed bump-and-hole engineering generates mutant glycosyltransferases that specifically accommodate bioorthogonal monosaccharides as an enabling technique of glycobiology. We review the groundbreaking discoveries that have led to applying the tactic in the living cell and the implications in the context of current developments in mass spectrometry glycoproteomics.
AU  - Cioce,A
AU  - Malaker,SA
AU  - Schumann,B
DO  - 10.1016/j.cbpa.2020.09.001
EP  - 78
PY  - 2021///
SN  - 1367-5931
SP  - 66
TI  - Generating orthogonal glycosyltransferase and nucleotide sugar pairs as next-generation glycobiology tools
T2  - Current Opinion in Chemical Biology
UR  - http://dx.doi.org/10.1016/j.cbpa.2020.09.001
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33125942
UR  - http://hdl.handle.net/10044/1/101477
VL  - 60
ER  -
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