Imperial College London
Alternate

Professor Brian G Spratt FRS

Faculty of Medicine, School of Public Health

Emeritus Professor
 
 
 
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Contact

 

+44 (0)20 7594 3625b.spratt

 
 
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Location

 

G30Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Boonsilp:2013:10.1371/journal.pntd.0001954,
author = {Boonsilp, S and Thaipadungpanit, J and Amornchai, P and Wuthiekanun, V and Bailey, MS and Holden, MTG and Zhang, C and Jiang, X and Koizumi, N and Taylor, K and Galloway, R and Hoffmaster, AR and Craig, S and Smythe, LD and Hartskeerl, RA and Day, NP and Chantratita, N and Feil, EJ and Aanensen, DM and Spratt, BG and Peacock, SJ},
doi = {10.1371/journal.pntd.0001954},
journal = {PLOS Neglected Tropical Diseases},
title = {A Single Multilocus Sequence Typing (MLST) scheme for seven athogenic Leptospira species},
url = {http://dx.doi.org/10.1371/journal.pntd.0001954},
volume = {7},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background:The availableLeptospiramultilocus sequence typing (MLST) scheme supported by a MLST website is limited toL. interrogansandL. kirschneri. Our aim was to broaden the utility of this scheme to incorporate a total of seven pathogenicspecies.Methodology and Findings:We modified the existing scheme by replacing one of the seven MLST loci (fadDwas changedtocaiB), as the former gene did not appear to be present in some pathogenic species. Comparison of the original andmodified schemes using data forL.  interrogansandL.  kirschneridemonstrated that the discriminatory power of the twoschemes was not significantly different. The modified scheme was used to further characterize 325 isolates (L.  alexanderi [n = 5], L. borgpetersenii[n = 34], L. interrogans [n = 222], L. kirschneri [n = 29], L. noguchii [n = 9], L. santarosai [n = 10], and L.weilii [n = 16]). Phylogenetic analysis using concatenated sequences of the 7 loci demonstrated that each speciescorresponded to a discrete clade, and that no strains were misclassified at the species level. Comparison between genotype and serovar was possible for 254 isolates. Of the 31 sequence types (STs) represented by at least two isolates, 18 STs included isolates assigned to two or three different serovars. Conversely, 14 serovars were identified that contained between 2 to 10 different STs. New observations were made on the global phylogeography ofLeptospira spp., and the utility of MLST in making associations between human disease and specific maintenance hosts was demonstrated.Conclusion:The new MLST scheme, supported by an updated MLST website, allows the characterization and species assignment of isolates of the seven major pathogenic species associated with leptospirosis.
AU  - Boonsilp,S
AU  - Thaipadungpanit,J
AU  - Amornchai,P
AU  - Wuthiekanun,V
AU  - Bailey,MS
AU  - Holden,MTG
AU  - Zhang,C
AU  - Jiang,X
AU  - Koizumi,N
AU  - Taylor,K
AU  - Galloway,R
AU  - Hoffmaster,AR
AU  - Craig,S
AU  - Smythe,LD
AU  - Hartskeerl,RA
AU  - Day,NP
AU  - Chantratita,N
AU  - Feil,EJ
AU  - Aanensen,DM
AU  - Spratt,BG
AU  - Peacock,SJ
DO  - 10.1371/journal.pntd.0001954
PY  - 2013///
SN  - 1935-2735
TI  - A Single Multilocus Sequence Typing (MLST) scheme for seven athogenic Leptospira species
T2  - PLOS Neglected Tropical Diseases
UR  - http://dx.doi.org/10.1371/journal.pntd.0001954
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000314360200005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/29992
VL  - 7
ER  -
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