Imperial College London


Faculty of Natural SciencesDepartment of Physics

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Publication Type

8 results found

Lagarto J, Dyer B, Talbot C, Peters N, French P, Lyon A, Dunsby Cet al., 2018, Characterization of NAD(P)H and FAD autofluorescence signatures in a Langendorff isolated-perfused rat heart model, Biomedical Optics Express, Vol: 9, Pages: 4978-4978, ISSN: 2156-7085

Autofluorescence spectroscopy is a promising label-free approach to characterize biological samples with demonstrated potential to report structural and biochemical alterations in tissues in a number of clinical applications. We report a characterization of the ex vivo autofluorescence fingerprint of cardiac tissue, exploiting a Langendorff-perfused isolated rat heart model to induce physiological insults to the heart, with a view to understanding how metabolic alterations affect the autofluorescence signals. Changes in the autofluorescence intensity and lifetime signatures associated with reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) were characterized during oxygen- or glucose-depletion protocols. Results suggest that both NAD(P)H and FAD autofluorescence intensity and lifetime parameters are sensitive to changes in the metabolic state of the heart owing to oxygen deprivation. We also observed changes in NAD(P)H fluorescence intensity and FAD lifetime parameter on reperfusion of oxygen, which might provide information on reperfusion injury, and permanent tissue damage or changes to the tissue during recovery from oxygen deprivation. We found that changes in the autofluorescence signature following glucose-depletion are, in general, less pronounced, and most clearly visible in NAD(P)H related parameters. Overall, the results reported in this investigation can serve as baseline for future investigations of cardiac tissue involving autofluorescence measurements.

Journal article

Dyer BT, Elder JM, Lagarto J, Harding SE, French PMW, Peters NS, Dunsby C, Lyon ARet al., 2015, Application of label-free autofluorescence lifetime in vivo to measure changes in myocardial fibrosis and metabolism in a doxorubicin cardiomyopathy heart failure model, Congress of the European-Society-of-Cardiology (ESC), Publisher: OXFORD UNIV PRESS, Pages: 151-151, ISSN: 0195-668X

Conference paper

Dyer BT, Elder JM, Lagarto J, Harding SE, French PMW, Peters NS, Dunsby C, Lyon ARet al., 2015, LABEL-FREE AUTOFLUORESCENCE LIFETIME TO ASSESS CHANGES IN MYOCARDIAL FIBROSIS AND METABOLISM IN VIVO IN A DOXORUBICIN CARDIOMYOPATHY HEART FAILURE MODEL, British-Cardiac-Society (BCS) Annual Conference on Hearts and Genes, Publisher: BMJ PUBLISHING GROUP, Pages: A94-A94, ISSN: 1355-6037

Conference paper

Dyer BTB, de Jesus Reis Lagarto J, Sikkel M, Mills A, French P, Peters NS, Dunsby C, Lyon ARet al., 2015, Application of label-free autofluorescence lifetime in vivo to measure changes in myocardial fibrosis and metabolism associated with myocardial infarction and heart failure, EUROPEAN JOURNAL OF HEART FAILURE, Vol: 17, Pages: 367-367, ISSN: 1388-9842

Journal article

Lagarto J, Dyer BT, Talbot C, Sikkel MB, Peters NS, French PMW, Lyon AR, Dunsby Cet al., 2015, Application of time-resolved autofluorescence to label-free in vivo optical mapping of changes in tissue matrix and metabolism associated with myocardial infarction and heart failure, Biomedical Optics Express, Vol: 6, Pages: 324-346, ISSN: 2156-7085

We investigate the potential of an instrument combining timeresolvedspectrofluorometry and diffuse reflectance spectroscopy tomeasure structural and metabolic changes in cardiac tissue in vivo in a 16week post-myocardial infarction heart failure model in rats. In the scarregion, we observed changes in the fluorescence signal that can beexplained by increased collagen content, which is in good agreement withhistology. In areas remote from the scar tissue, we measured changes in thefluorescence signal (p < 0.001) that cannot be explained by differences incollagen content and we attribute this to altered metabolism within themyocardium. A linear discriminant analysis algorithm was applied to themeasurements to predict the tissue disease state. When we combine allmeasurements, our results reveal high diagnostic accuracy in the infarctedarea (100%) and border zone (94.44%) as well as in remote regions fromthe scar (> 77%). Overall, our results demonstrate the potential of ourinstrument to characterize structural and metabolic changes in a failing heartin vivo without using exogenous labels.

Journal article

Dyer BT, Lagarto J, French P, Peters NS, Dunsby C, Lyon ARet al., 2014, TIME-RESOLVED AUTOFLUORESCENCE SPECTROSCOPY AS LABEL-FREE METHOD TO CHARACTERISE ACUTE CHANGES IN EX VIVO MODELS OF CARDIAC DISEASE, Autumn Meeting of the British-Society-for-Cardiovascular-Research (BSCR) on Cardiovascular Signalling in Health and Disease, Publisher: BMJ PUBLISHING GROUP, ISSN: 1355-6037

Conference paper

Kelly DJ, Warren SC, Kumar S, Lagarto JL, Dyer BT, Margineanu A, Lam EW-F, Dunsby C, French PMWet al., 2014, An automated multiwell plate reading film microscope for live cell autofluorescence lifetime assays, JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES, Vol: 7, ISSN: 1793-5458

Journal article

Dyer BT, Martin JL, Mitchell JL, Sauven NC, Gazzard Bet al., 2004, Hypokalaemia in ibuprofen and codeine phosphate abuse, INTERNATIONAL JOURNAL OF CLINICAL PRACTICE, Vol: 58, Pages: 1061-1062, ISSN: 1368-5031

Journal article

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