Imperial College London
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DrChristopherAylett

Faculty of Medicine, Department of Infectious Disease

Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 3862c.aylett

 
 
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Location

 

6.40bFlowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{deYMartín:2021:nar/gkab539,
author = {deYMartín, Garrido N and Orekhova, M and Lai, Wan Loong YTE and Litvinova, A and Ramlaul, K and Artamonova, T and Melnikov, AS and Serdobintsev, P and Aylett, CHS and Yakunina, M},
doi = {nar/gkab539},
journal = {Nucleic Acids Research},
pages = {7732--7739},
title = {Structure of the bacteriophage PhiKZ non-virion RNA polymerase},
url = {http://dx.doi.org/10.1093/nar/gkab539},
volume = {49},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bacteriophage ΦKZ (PhiKZ) is the archetype of a family of massive bacterial viruses. It is considered to have therapeutic potential as its host, Pseudomonas aeruginosa, is an opportunistic, intrinsically antibiotic resistant, pathogen that kills tens of thousands worldwide each year. ΦKZ is an incredibly interesting virus, expressing many systems that the host already possesses. On infection, it forms a ‘nucleus’, erecting a barrier around its genome to exclude host endonucleases and CRISPR-Cas systems. ΦKZ infection is independent of the host transcriptional apparatus. It expresses two different multi-subunit RNA polymerases (RNAPs): the virion RNAP (vRNAP) is injected with the viral DNA during infection to transcribe early genes, including those encoding the non-virion RNAP (nvRNAP), which transcribes all further genes. ΦKZ nvRNAP is formed by four polypeptides thought to represent homologues of the eubacterial β/β′ subunits, and a fifth with unclear homology, but essential for transcription. We have resolved the structure of ΦKZ nvRNAP to better than 3.0 Å, shedding light on its assembly, homology, and the biological role of the fifth subunit: it is an embedded, integral member of the complex, the position, structural homology and biochemical role of which imply that it has evolved from an ancestral homologue to σ-factor.
AU  - deYMartín,Garrido N
AU  - Orekhova,M
AU  - Lai,Wan Loong YTE
AU  - Litvinova,A
AU  - Ramlaul,K
AU  - Artamonova,T
AU  - Melnikov,AS
AU  - Serdobintsev,P
AU  - Aylett,CHS
AU  - Yakunina,M
DO  - nar/gkab539
EP  - 7739
PY  - 2021///
SN  - 0305-1048
SP  - 7732
TI  - Structure of the bacteriophage PhiKZ non-virion RNA polymerase
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gkab539
UR  - https://academic.oup.com/nar/article/49/13/7732/6310793
UR  - http://hdl.handle.net/10044/1/90331
VL  - 49
ER  -
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