Imperial College London
Alternate

ProfessorCharlesBangham

Institute of Infection

Co-Director of the Institute of Infection
 
 
 
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Contact

 

+44 (0)20 7594 3730c.bangham Website

 
 
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Assistant

 

Ms Linda Hollick +44 (0)20 7594 3729

 
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Location

 

115Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Katsuya:2022:infdis/jiab202,
author = {Katsuya, H and Cook, LBM and Rowan, AG and Melamed, A and Turpin, J and Ito, J and Islam, S and Miyazato, P and Jek, Yang Tan B and Matsuo, M and Miyakawa, T and Nakata, H and Matsushita, S and Taylor, GP and Bangham, CRM and Kimura, S and Satou, Y},
doi = {infdis/jiab202},
journal = {Journal of Infectious Diseases},
pages = {317--326},
title = {Clonality of HIV-1 and HTLV-1 infected cells in naturally coinfected individuals},
url = {http://dx.doi.org/10.1093/infdis/jiab202},
volume = {225},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Coinfection with HIV-1 and HTLV-1 diminishes the value of the CD4 + T-cell count in diagnosing AIDS, and increases the rate of HTLV-1-associated myelopathy. It remains elusive how HIV-1/HTLV-1 coinfection is related to such clinical characteristics. Here, we investigated the mutual effect of HIV-1/HTLV-1 coinfection on their integration sites (ISs) and the clonal expansion. METHODS: We extracted DNA from longitudinal peripheral blood samples from 7 HIV-1/HTLV-1 coinfected individuals, and from 12 HIV-1 and 13 HTLV-1 mono-infected individuals. The proviral loads (PVL) were quantified using real-time PCR. Viral ISs and clonality were quantified by ligation-mediated PCR followed by high-throughput sequencing. RESULTS: The PVL of both HIV-1 and HTLV-1 in coinfected individuals was significantly higher than that of the respective virus in mono-infected individuals. The degree of oligoclonality of both HIV-1- and HTLV-1-infected cells in co-infected individuals was also greater than that in mono-infected subjects. The ISs of HIV-1 in cases of coinfection were more frequently located in intergenic regions and transcriptionally silent regions, compared with HIV-1 mono-infected individuals. CONCLUSION: HIV-1/HTLV-1 coinfection makes an impact on the distribution of viral ISs and the clonality of virus-infected cells and thus may alter the risks of both HTLV-1- and HIV-1-associated disease.
AU  - Katsuya,H
AU  - Cook,LBM
AU  - Rowan,AG
AU  - Melamed,A
AU  - Turpin,J
AU  - Ito,J
AU  - Islam,S
AU  - Miyazato,P
AU  - Jek,Yang Tan B
AU  - Matsuo,M
AU  - Miyakawa,T
AU  - Nakata,H
AU  - Matsushita,S
AU  - Taylor,GP
AU  - Bangham,CRM
AU  - Kimura,S
AU  - Satou,Y
DO  - infdis/jiab202
EP  - 326
PY  - 2022///
SN  - 0022-1899
SP  - 317
TI  - Clonality of HIV-1 and HTLV-1 infected cells in naturally coinfected individuals
T2  - Journal of Infectious Diseases
UR  - http://dx.doi.org/10.1093/infdis/jiab202
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33844021
UR  - https://academic.oup.com/jid/advance-article/doi/10.1093/infdis/jiab202/6222242
UR  - http://hdl.handle.net/10044/1/88183
VL  - 225
ER  -
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