Imperial College London
Alternate

ProfessorCharlesBangham

Institute of Infection

Co-Director of the Institute of Infection
 
 
 
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Contact

 

+44 (0)20 7594 3730c.bangham Website

 
 
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Assistant

 

Ms Linda Hollick +44 (0)20 7594 3729

 
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Location

 

115Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Satou:2017:10.1038/s41598-017-07307-4,
author = {Satou, Y and Katsuya, H and Fukuda, A and Misawa, N and Ito, J and Uchiyama, Y and Miyazato, P and Islam, S and Fassati, A and Melamed, A and Bangham, CRM and Koyanagi, Y and Sato, K},
doi = {10.1038/s41598-017-07307-4},
journal = {Scientific Reports},
title = {Dynamics and mechanisms of clonal expansion of HIV-1-infected cells in a humanized mouse model.},
url = {http://dx.doi.org/10.1038/s41598-017-07307-4},
volume = {7},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Combination anti-retroviral therapy (cART) has drastically improved the clinical outcome of HIV-1 infection. Nonetheless, despite effective cART, HIV-1 persists indefinitely in infected individuals. Clonal expansion of HIV-1-infected cells in peripheral blood has been reported recently. cART is effective in stopping the retroviral replication cycle, but not in inhibiting clonal expansion of the infected host cells. Thus, the proliferation of HIV-1-infected cells may play a role in viral persistence, but little is known about the kinetics of the generation, the tissue distribution or the underlying mechanism of clonal expansion in vivo. Here we analyzed the clonality of HIV-1-infected cells using high-throughput integration site analysis in a hematopoietic stem cell-transplanted humanized mouse model. Clonally expanded, HIV-1-infected cells were detectable at two weeks post infection, their abundance increased with time, and certain clones were present in multiple organs. Expansion of HIV-1-infected clones was significantly more frequent when the provirus was integrated near host genes in specific gene ontological classes, including cell activation and chromatin regulation. These results identify potential drivers of clonal expansion of HIV-1-infected cells in vivo.
AU  - Satou,Y
AU  - Katsuya,H
AU  - Fukuda,A
AU  - Misawa,N
AU  - Ito,J
AU  - Uchiyama,Y
AU  - Miyazato,P
AU  - Islam,S
AU  - Fassati,A
AU  - Melamed,A
AU  - Bangham,CRM
AU  - Koyanagi,Y
AU  - Sato,K
DO  - 10.1038/s41598-017-07307-4
PY  - 2017///
SN  - 2045-2322
TI  - Dynamics and mechanisms of clonal expansion of HIV-1-infected cells in a humanized mouse model.
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-017-07307-4
UR  - https://www.ncbi.nlm.nih.gov/pubmed/28761140
UR  - http://hdl.handle.net/10044/1/50048
VL  - 7
ER  -
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