Imperial College London
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ProfessorChristopherChiu

Faculty of Medicine, Department of Infectious Disease

Professor of Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 3313 2301c.chiu Website

 
 
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Location

 

8N.15Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Schott:2022:10.1016/j.xgen.2022.100207,
author = {Schott, BH and Wang, L and Zhu, X and Harding, AT and Ko, ER and Bourgeois, JS and Washington, EJ and Burke, TW and Anderson, J and Bergstrom, E and Gardener, Z and Paterson, S and Brennan, RG and Chiu, C and McClain, MT and Woods, CW and Gregory, SG and Heaton, NS and Ko, DC},
doi = {10.1016/j.xgen.2022.100207},
journal = {Cell Genomics},
title = {Single-cell genome-wide association reveals a nonsynonymous variant in ERAP1 confers increased susceptibility to influenza virus},
url = {http://dx.doi.org/10.1016/j.xgen.2022.100207},
volume = {2},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - During pandemics, individuals exhibit differences in risk and clinical outcomes. Here, we developed single-cell high-throughput human in vitro susceptibility testing (scHi-HOST), a method for rapidly identifying genetic variants that confer resistance and susceptibility. We applied this method to influenza A virus (IAV), the cause of four pandemics since the start of the 20th century. scHi-HOST leverages single-cell RNA sequencing (scRNA-seq) to simultaneously assign genetic identity to cells in mixed infections of cell lines of European, African, and Asian origin, reveal associated genetic variants for viral burden, and identify expression quantitative trait loci. Integration of scHi-HOST with human challenge and experimental validation demonstrated that a missense variant in endoplasmic reticulum aminopeptidase 1 (ERAP1; rs27895) increased IAV burden in cells and human volunteers. rs27895 exhibits population differentiation, likely contributing to greater permissivity of cells from African populations to IAV. scHi-HOST is a broadly applicable method and resource for decoding infectious-disease genetics.
AU  - Schott,BH
AU  - Wang,L
AU  - Zhu,X
AU  - Harding,AT
AU  - Ko,ER
AU  - Bourgeois,JS
AU  - Washington,EJ
AU  - Burke,TW
AU  - Anderson,J
AU  - Bergstrom,E
AU  - Gardener,Z
AU  - Paterson,S
AU  - Brennan,RG
AU  - Chiu,C
AU  - McClain,MT
AU  - Woods,CW
AU  - Gregory,SG
AU  - Heaton,NS
AU  - Ko,DC
DO  - 10.1016/j.xgen.2022.100207
PY  - 2022///
SN  - 2666-979X
TI  - Single-cell genome-wide association reveals a nonsynonymous variant in ERAP1 confers increased susceptibility to influenza virus
T2  - Cell Genomics
UR  - http://dx.doi.org/10.1016/j.xgen.2022.100207
UR  - http://hdl.handle.net/10044/1/100790
VL  - 2
ER  -
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