Imperial College London
Alternate

Emeritus ProfessorCharlesCoutelle

Faculty of Medicine, National Heart & Lung Institute

Emeritus Professor
 
 
 
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Contact

 

+44 (0)7906 614 491c.coutelle

 
 
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Location

 

Open SpaceSir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{David:2010:10.1016/S1028-4559(10)60004-6,
author = {David, AL and Abi-Nader, KN and Weisz, B and Shaw, SWS and Themis, M and Cook, T and Coutelle, C and Rodeck, CH and Peebles, DM},
doi = {10.1016/S1028-4559(10)60004-6},
journal = {Taiwan J Obstet Gynecol},
pages = {23--29},
title = {Ultrasonographic development of the fetal sheep stomach and evaluation of early gestation ultrasound-guided in utero intragastric injection.},
url = {http://dx.doi.org/10.1016/S1028-4559(10)60004-6},
volume = {49},
year = {2010}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - OBJECTIVE: Safely targeting the fetal gastrointestinal tract during early gestation is essential to develop effective prenatal gene therapy for gastrointestinal diseases. In this study, we aimed to characterize the development of the fetal sheep stomach sonographically and to determine the optimum gestational age, as well as the shortterm morbidity and mortality of early-gestation ultrasound-guided intragastric injection. MATERIALS AND METHODS: In experiments investigating ultrasound-guided prenatal gene therapy, we studied the size and development of the stomach of 185 sheep fetuses (33-144 days' gestational age [GA]; term is 145 days). Ultrasound-guided intragastric injection was performed in 12 fetuses at 55-62 days' GA and postmortem examinations were performed 48 hours later. RESULTS: The stomach was not visible at or before 40 days' GA, but it was seen in all fetuses at 55 days' GA or more. The anteroposterior, transverse and longitudinal diameters of the stomach increased in a quasi-linear fashion throughout gestation. Intragastric injection was successful in 10 out of the 11 fetuses (91%) injected at 60-62 days' GA, with nine fetuses (91%) surviving this procedure. CONCLUSION: In the early-gestation sheep fetus, ultrasound-guided intragastric injection has a good success rate with a low short-term mortality and morbidity.
AU  - David,AL
AU  - Abi-Nader,KN
AU  - Weisz,B
AU  - Shaw,SWS
AU  - Themis,M
AU  - Cook,T
AU  - Coutelle,C
AU  - Rodeck,CH
AU  - Peebles,DM
DO  - 10.1016/S1028-4559(10)60004-6
EP  - 29
PY  - 2010///
SP  - 23
TI  - Ultrasonographic development of the fetal sheep stomach and evaluation of early gestation ultrasound-guided in utero intragastric injection.
T2  - Taiwan J Obstet Gynecol
UR  - http://dx.doi.org/10.1016/S1028-4559(10)60004-6
UR  - https://www.ncbi.nlm.nih.gov/pubmed/20466288
VL  - 49
ER  -
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