Publications
279 results found
Sweaad WK, Stefanizzi FM, Chamorro-Jorganes A, et al., 2021, Relevance of N6-methyladenosine regulators for transcriptome: Implications for development and the cardiovascular system, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 160, Pages: 56-70, ISSN: 0022-2828
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- Citations: 4
Scott A, Ballesteros LS, Bradshaw M, et al., 2021, In Vivo Characterization of Endogenous Cardiovascular Extracellular Vesicles in Larval and Adult Zebrafish, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 41, Pages: 2454-2468, ISSN: 1079-5642
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- Citations: 14
Aday S, Hazan-Halevy I, Chamorro-Jorganes A, et al., 2021, Bioinspired artificial exosomes based on lipid nanoparticles carrying let-7b-5p promote angiogenesis in vitro and in vivo, Molecular Therapy, Vol: 29, Pages: 2239-2252, ISSN: 1525-0016
MicroRNAs (miRNAs) regulate gene expression by post-transcriptional inhibition of target genes. Proangiogenic small extracellular vesicles (sEVs; popularly identified with the name "exosomes") with a composite cargo of miRNAs are secreted by cultured stem cells and present in human biological fluids. Lipid nanoparticles (LNPs) represent an advanced platform for clinically approved delivery of RNA therapeutics. In this study, we aimed to (1) identify the miRNAs responsible for sEV-induced angiogenesis; (2) develop the prototype of bioinspired "artificial exosomes" (AEs) combining LNPs with a proangiogenic miRNA, and (3) validate the angiogenic potential of the bioinspired AEs. We previously reported that human sEVs from bone marrow (BM)-CD34+ cells and pericardial fluid (PF) are proangiogenic. Here, we have shown that sEVs secreted from saphenous vein pericytes and BM mesenchymal stem cells also promote angiogenesis. Analysis of miRNA datasets available in-house or datamined from GEO identified the let-7 family as common miRNA signature of the proangiogenic sEVs. LNPs with either hsa-let-7b-5p or cyanine 5 (Cy5)-conjugated Caenorhabditis elegans miR-39 (Cy5-cel-miR-39; control miRNA) were prepared using microfluidic micromixing. let-7b-5p-AEs did not cause toxicity and transferred functionally active let-7b-5p to recipient endothelial cells (ECs). let-7b-AEs also improved EC survival under hypoxia and angiogenesis in vitro and in vivo. Bioinspired proangiogenic AEs could be further developed into innovative nanomedicine products targeting ischemic diseases.
Badimon L, Robinson EL, Jusic A, et al., 2021, Cardiovascular RNA markers and artificial intelligence may improve COVID-19 outcome: a position paper from the EU-CardioRNA COST Action CA17129, CARDIOVASCULAR RESEARCH, Vol: 117, Pages: 1823-1840, ISSN: 0008-6363
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- Citations: 11
Chamorro-Jorganes A, Sweaad WK, Katare R, et al., 2021, METTL3 Regulates Angiogenesis by Modulating let-7e-5p and miRNA-18a-5p Expression in Endothelial Cells, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 41, Pages: E325-E337, ISSN: 1079-5642
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- Citations: 23
Moscarelli M, Angelini GD, Emanueli C, et al., 2021, Remote ischemic preconditioning in isolated valve intervention. A pooled meta-analysis, INTERNATIONAL JOURNAL OF CARDIOLOGY, Vol: 324, Pages: 146-151, ISSN: 0167-5273
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- Citations: 1
Bollini S, Emanueli C, 2021, To serve and protect: a new heart patrolling and recycling role for macrophages, CARDIOVASCULAR RESEARCH, Vol: 117, Pages: E17-E20, ISSN: 0008-6363
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- Citations: 2
de Abreu RC, Fernandes H, Martins PADC, et al., 2020, Native and bioengineered extracellular vesicles for cardiovascular therapeutics, NATURE REVIEWS CARDIOLOGY, Vol: 17, Pages: 685-697, ISSN: 1759-5002
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- Citations: 170
Nicastro L, Calapano F, Nunez Toldra R, et al., 2020, Mechanical load modulates the cargo of secreted extracellular vesicles (EVs) from living myocardial slices, European-Society-of-Cardiology (ESC) Congress, Publisher: OXFORD UNIV PRESS, Pages: 3614-3614, ISSN: 0195-668X
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- Citations: 1
Greco S, Made A, Gaetano C, et al., 2020, Noncoding RNAs implication in cardiovascular diseases in the COVID-19 era, JOURNAL OF TRANSLATIONAL MEDICINE, Vol: 18
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- Citations: 11
Spencer HL, Sanders R, Boulberdaa M, et al., 2020, The LINC00961 transcript and its encoded micropeptide, small regulatory polypeptide of amino acid response, regulate endothelial cell function, CARDIOVASCULAR RESEARCH, Vol: 116, Pages: 1981-1994, ISSN: 0008-6363
Robinson EL, Gomes CPC, Potocnjak I, et al., 2020, A Year in the Life of the EU-CardioRNA COST Action: CA17129 Catalysing Transcriptomics Research in Cardiovascular Disease, NON-CODING RNA, Vol: 6
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- Citations: 2
Shearn AIU, Aday S, Ben-Aicha S, et al., 2020, Analysis of neat biofluids obtained during cardiac surgery using nanoparticle tracking analysis: methodological considerations, Frontiers in Cell and Developmental Biology, Vol: 8, Pages: 1-14, ISSN: 2296-634X
Small extracellular vesicles (sEVs) are those nanovesicles 30–150 nm in size with a role in cell signalling and potential as biomarkers of disease. Nanoparticle tracking analysis (NTA) techniques are commonly used to measure sEV concentration in biofluids. However, this quantification technique can be susceptible to sample handing and machine settings. Moreover, some classes of lipoproteins are of similar sizes and could therefore confound sEV quantification, particularly in blood-derived preparations, such serum and plasma. Here we have provided methodological information on NTA measurements and systematically investigated potential factors that could interfere with the reliability and repeatability of results obtained when looking at neat biofluids (i.e., human serum and pericardial fluid) obtained from patients undergoing cardiac surgery and from healthy controls. Data suggest that variables that can affect vesicle quantification include the level of contamination from lipoproteins, number of sample freeze/thaw cycles, sample filtration, using saline-based diluents, video length and keeping the number of particles per frame within defined limits. Those parameters that are of less concern include focus, the “Maximum Jump” setting and the number of videos recorded. However, if these settings are clearly inappropriate the results obtained will be spurious. Similarly, good experimental practice suggests that multiple videos should be recorded. In conclusion, NTA is a perfectible, but still commonly used system for sEVs analyses. Provided users handle their samples with a highly robust and consistent protocol, and accurately report these aspects, they can obtain data that could potentially translate into new clinical biomarkers for diagnosis and monitoring of cardiovascular disease.
Emanueli C, Badimon L, Martelli F, et al., 2020, Call to action for the cardiovascular side of COVID-19 A call for cooperative action from the EU-CardioRNA COST Action, EUROPEAN HEART JOURNAL, Vol: 41, Pages: 1796-1797, ISSN: 0195-668X
Ozaki Tan SJ, Floriano JF, Nicastro L, et al., 2020, Novel Applications of Mesenchymal Stem Cell-Derived Exosomes for Myocardial Infarction Therapeutics, BIOMOLECULES, Vol: 10
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- Citations: 45
Vesentini G, Barbosa AMP, Damasceno DC, et al., 2020, Alterations in the structural characteristics of rectus abdominis muscles caused by diabetes and pregnancy: A comparative study of the rat model and women, PLOS ONE, Vol: 15, ISSN: 1932-6203
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- Citations: 11
Stoica SC, Dorobantu DM, Vardeu A, et al., 2020, MicroRNAs as potential biomarkers in congenital heart surgery, JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, Vol: 159, Pages: 1532-+, ISSN: 0022-5223
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- Citations: 8
Tikhomirov R, Donnell BR-O, Catapano F, et al., 2020, Exosomes: From potential culprits to new therapeutic promise in the setting of cardiac fibrosis, Cells, Vol: 9, ISSN: 2073-4409
Fibrosis is a significant global health problem associated with many inflammatory and degenerative diseases affecting multiple organs, individually or simultaneously. Fibrosis develops when extracellular matrix (ECM) remodeling becomes excessive or uncontrolled and is associated with nearly all forms of heart disease. Cardiac fibroblasts and myofibroblasts are the main effectors of ECM deposition and scar formation. The heart is a complex multicellular organ, where the various resident cell types communicate between themselves and with cells of the blood and immune systems. Exosomes, which are small extracellular vesicles, (EVs), contribute to cell-to-cell communication and their pathophysiological relevance and therapeutic potential is emerging. Here, we will critically review the role of endogenous exosomes as possible fibrosis mediators and discuss the possibility of using stem cell-derived and/or engineered exosomes as anti-fibrotic agents.
Marchetti M, Meloni M, Anwar M, et al., 2020, MicroRNA-24-3p Targets Notch and Other Vascular Morphogens to Regulate Post-ischemic Microvascular Responses in Limb Muscles, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol: 21
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- Citations: 14
Floriano JF, Willis G, Catapano F, et al., 2020, Exosomes Could Offer New Options to Combat the Long-Term Complications Inflicted by Gestational Diabetes Mellitus, CELLS, Vol: 9
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- Citations: 17
Gomes CPDC, Schroen B, Kuster GM, et al., 2020, Regulatory RNAs in Heart Failure, CIRCULATION, Vol: 141, Pages: 313-328, ISSN: 0009-7322
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- Citations: 100
Chamorro-Jorganes A, Anwar M, Emanueli C, 2019, Changes in HDL-microRNA might create a lasting memory of high-fat diet, Cardiovascular Research, Vol: 116, Pages: 1237-1239, ISSN: 0008-6363
Dixit P, Anwar M, Saif J, et al., 2019, Impaired secretion of clusterin in pericardial fluid of diabetics, a deleterious outcome for the cardiac micro-vasculature, Congress of the European-Society-of-Cardiology (ESC) / World Congress of Cardiology, Publisher: OXFORD UNIV PRESS, Pages: 3912-3912, ISSN: 0195-668X
Chen H, Moreno-Moral A, Pesce F, et al., 2019, Author Correction: WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling, Nature Communications, Vol: 10, ISSN: 2041-1723
Chen H, Moreno-Moral A, Pesce F, et al., 2019, WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling, Nature Communications, Vol: 10, Pages: 1-19, ISSN: 2041-1723
Cardiac fibrosis is a final common pathology in inherited and acquired heart diseases that causes cardiac electrical and pump failure. Here, we use systems genetics to identify a pro-fibrotic gene network in the diseased heart and show that this network is regulated by the E3 ubiquitin ligase WWP2, specifically by the WWP2-N terminal isoform. Importantly, the WWP2-regulated pro-fibrotic gene network is conserved across different cardiac diseases characterized by fibrosis: human and murine dilated cardiomyopathy and repaired tetralogy of Fallot. Transgenic mice lacking the N-terminal region of the WWP2 protein show improved cardiac function and reduced myocardial fibrosis in response to pressure overload or myocardial infarction. In primary cardiac fibroblasts, WWP2 positively regulates the expression of pro-fibrotic markers and extracellular matrix genes. TGFβ1 stimulation promotes nuclear translocation of the WWP2 isoforms containing the N-terminal region and their interaction with SMAD2. WWP2 mediates the TGFβ1-induced nucleocytoplasmic shuttling and transcriptional activity of SMAD2.
Aday S, Halevy I, Anwar M, et al., 2019, Development of Bioinspired Synthetic Exosomes With Proangiogenic Potential, 14th Annual American-Heart-Association's Basic Cardiovascular Sciences (BCVS) Scientific Sessions - Integrative Approaches to Complex Cardiovascular Diseases, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7330
Sweaad WK, Chamorro-Jorganes A, Besnier M, et al., 2019, m6A RNA methylation regulates angiogenesis and is deregulated in diabetic endothelial cells: A possible new target for gene therapy of diabetic microangiopathy?, Annual Conference of the British-Society-for-Gene-and-Cell-Therapy, Publisher: MARY ANN LIEBERT, INC, Pages: A29-A30, ISSN: 1043-0342
Machado MJC, Boardman R, Riu F, et al., 2019, Enhanced notch signaling modulates unproductive revascularization in response to nitric oxide-angiopoietin signaling in a mouse model of peripheral ischemia, MICROCIRCULATION, Vol: 26, ISSN: 1073-9688
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- Citations: 3
Dang Z, Avolio E, Albertario A, et al., 2019, Nerve growth factor gene therapy improves bone marrow sensory innervation and nociceptor-mediated stem cell release in a mouse model of type 1 diabetes with limb ischaemia, DIABETOLOGIA, Vol: 62, Pages: 1297-1311, ISSN: 0012-186X
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- Citations: 12
Gomes CPDC, Agg B, Andova A, et al., 2019, Catalyzing Transcriptomics Research in Cardiovascular Disease: The CardioRNA COST Action CA17129, NON-CODING RNA, Vol: 5
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- Citations: 8
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