Publications
279 results found
Beltrami C, Shantikumar S, Laftha A, et al., 2016, The human pericardial fluid is enriched with cardiovascular-expressed microRNAs and exosomes with therapeutic potential, CARDIOVASCULAR RESEARCH, Vol: 111, Pages: S48-S48, ISSN: 0008-6363
Aday S, Besnier M, Zoldan J, et al., 2016, MICRORNA-17 AS THE TARGET OF IMMOBILIZED VASCULAR ENDOTHELIAL GROWTH FACTOR IN ENDOTHELIAL CELL SURVIVAL UNDER ISCHAEMIC CONDITIONS, Annual Conference of the British-Cardiovascular-Society (BCS) on Prediction and Prevention, Publisher: BMJ PUBLISHING GROUP, Pages: A133-A134, ISSN: 1355-6037
Fortunato TM, Beltrami C, Emanueli C, et al., 2016, Platelet lysate gel and endothelial progenitors stimulate microvascular network formation <i>in vitro</i>: tissue engineering implications, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322
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- Citations: 44
Emanueli C, Shearn AIU, Laftah A, et al., 2016, Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery, PLOS One, Vol: 11, ISSN: 1932-6203
IntroductionExosome nanoparticles carry a composite cargo, including microRNAs (miRs). Cultured cardiovascular cells release miR-containing exosomes. The exosomal trafficking of miRNAs from the heart is largely unexplored. Working on clinical samples from coronary-artery by-pass graft (CABG) surgery, we investigated if: 1) exosomes containing cardiac miRs and hence putatively released by cardiac cells increase in the circulation after surgery; 2) circulating exosomes and exosomal cardiac miRs correlate with cardiac troponin (cTn), the current “gold standard” surrogate biomarker of myocardial damage.Methods and ResultsThe concentration of exosome-sized nanoparticles was determined in serial plasma samples. Cardiac-expressed (miR-1, miR-24, miR-133a/b, miR-208a/b, miR-210), non-cardiovascular (miR-122) and quality control miRs were measured in whole plasma and in plasma exosomes. Linear regression analyses were employed to establish the extent to which the circulating individual miRs, exosomes and exosomal cardiac miR correlated with cTn-I. Cardiac-expressed miRs and the nanoparticle number increased in the plasma on completion of surgery for up to 48 hours. The exosomal concentration of cardiac miRs also increased after CABG. Cardiac miRs in the whole plasma did not correlate significantly with cTn-I. By contrast cTn-I was positively correlated with the plasma exosome level and the exosomal cardiac miRs.ConclusionsThe plasma concentrations of exosomes and their cargo of cardiac miRs increased in patients undergoing CABG and were positively correlated with hs-cTnI. These data provide evidence that CABG induces the trafficking of exosomes from the heart to the peripheral circulation. Future studies are necessary to investigate the potential of circulating exosomes as clinical biomarkers in cardiac patients.
Boulberdaa M, Scott E, Ballantyne M, et al., 2016, A Role for the Long Noncoding RNA SENCR in Commitment and Function of Endothelial Cells, Molecular Therapy, Vol: 24, Pages: 978-990, ISSN: 1525-0024
Despite the increasing importance of long noncoding RNA in physiology and disease, their role in endothelial biology remains poorly understood. Growing evidence has highlighted them to be essential regulators of human embryonic stem cell differentiation. SENCR, a vascular-enriched long noncoding RNA, overlaps the Friend Leukemia Integration virus 1 (FLI1) gene, a regulator of endothelial development. Therefore, we wanted to test the hypothesis that SENCR may contribute to mesodermal and endothelial commitment as well as in endothelial function. We thus developed new differentiation protocols allowing generation of endothelial cells from human embryonic stem cells using both directed and hemogenic routes. The expression of SENCR was markedly regulated during endothelial commitment using both protocols. SENCR did not control the pluripotency of pluripotent cells; however its overexpression significantly potentiated early mesodermal and endothelial commitment. In human umbilical endothelial cell (HUVEC), SENCR induced proliferation, migration, and angiogenesis. SENCR expression was altered in vascular tissue and cells derived from patients with critical limb ischemia and premature coronary artery disease compared to controls. Here, we showed that SENCR contributes to the regulation of endothelial differentiation from pluripotent cells and controls the angiogenic capacity of HUVEC. These data give novel insight into the regulatory processes involved in endothelial development and function.
Zakkar M, Angelini GD, Emanueli C, 2016, Regulation of Vascular Endothelium Inflammatory Signalling by Shear Stress., Current Vascular Pharmacology, Vol: 14, Pages: 181-186, ISSN: 1875-6212
The vascular endothelium plays a pivotal role in regulating vascular homeostasis. Blood flow exerts several mechanical forces on the luminal surface of the Endothelial Cell (EC) including pressure, circumferential stretch, and shear stress. It is widely believed that shear stress plays a central role in regulating EC inflammatory responses and the pathogenesis of atherosclerosis. High shear stress can induce an antiinflammatory status in EC, which is partially mediated by the production of proteins and transcription factors able to suppress different proinflammatory signalling pathways. In this review, we summarise the available evidence regarding the effect of shear stress on vascular EC and smooth muscle cells, the regulation of MAPK and NF-κB including the production of different negative regulators of inflammation such as MKP-1 and NRF2, and the production of microRNAs. We also discuss the possible links between shear stress and the development of atherosclerosis.
Munasinghe PE, Riu F, Dixit P, et al., 2016, Type-2 diabetes increases autophagy in the human heart through promotion of Beclin-1 mediated pathway, INTERNATIONAL JOURNAL OF CARDIOLOGY, Vol: 202, Pages: 13-20, ISSN: 0167-5273
Smith T, Rajakaruna C, Caputo M, et al., 2015, MicroRNAs in congenital heart disease, ANNALS OF TRANSLATIONAL MEDICINE, Vol: 3, ISSN: 2305-5839
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- Citations: 63
Meloni M, Cesselli D, Caporali A, et al., 2015, Cardiac Nerve Growth Factor Overexpression Induces Bone Marrow-derived Progenitor Cells Mobilization and Homing to the Infarcted Heart, MOLECULAR THERAPY, Vol: 23, Pages: 1854-1866, ISSN: 1525-0016
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- Citations: 12
Beltrami C, Angelini TG, Emanueli C, 2015, Noncoding RNAs in diabetes vascular complications, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 89, Pages: 42-50, ISSN: 0022-2828
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- Citations: 55
Munasinghe PE, Riu F, Dixit P, et al., 2015, Data supporting the activation of autophagy genes in the diabetic heart, DATA IN BRIEF, Vol: 5, Pages: 269-275, ISSN: 2352-3409
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- Citations: 5
Varadarajan S, Chen G-F, Das A, et al., 2015, Antioxidant-1, a Copper Transport Protein, promotes Inflammatory Neovascularization via Chaperone and Transcription Factor Function, Scientific Sessions and Resuscitation Science Symposium of the American-Heart-Association (AHA), Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Dang Z, Maselli D, Spinetti G, et al., 2015, Sensory neuropathy hampers nociception-mediated bone marrow stem cell release in mice and patients with diabetes, DIABETOLOGIA, Vol: 58, Pages: 2653-2662, ISSN: 0012-186X
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- Citations: 31
Chen G-F, Sudhahar V, Youn S-W, et al., 2015, Copper Transport Protein Antioxidant-1 Promotes Inflammatory Neovascularization via Chaperone and Transcription Factor Function, SCIENTIFIC REPORTS, Vol: 5, ISSN: 2045-2322
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- Citations: 46
Besnier M, Meloni M, Caporali A, et al., 2015, IL-33/ST2 in angiogenesis and limb ischemia in mice, Congress of the European-Society-of-Cardiology (ESC), Publisher: OXFORD UNIV PRESS, Pages: 1117-1117, ISSN: 0195-668X
Caporali A, Meloni M, Nailor A, et al., 2015, p75(NTR)-dependent activation of NF-kappa B regulates microRNA-503 transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia, Nature Communications, Vol: 6, Pages: 1-13, ISSN: 2041-1723
The communication between vascular endothelial cells (ECs) and pericytes in the microvasculature is fundamental for vascular growth and homeostasis; however, these processes are disrupted by diabetes. Here we show that modulation of p75NTR expression in ECs exposed to high glucose activates transcription of miR-503, which negatively affects pericyte function. p75NTR activates NF-κB to bind the miR-503 promoter and upregulate miR-503 expression in ECs. NF-κB further induces activation of Rho kinase and shedding of endothelial microparticles carrying miR-503, which transfer miR-503 from ECs to vascular pericytes. The integrin-mediated uptake of miR-503 in the recipient pericytes reduces expression of EFNB2 and VEGFA, resulting in impaired migration and proliferation. We confirm operation of the above mechanisms in mouse models of diabetes, in which EC-derived miR-503 reduces pericyte coverage of capillaries, increased permeability and impaired post-ischaemic angiogenesis in limb muscles. Collectively, our data demonstrate that miR-503 regulates pericyte–endothelial crosstalk in microvascular diabetic complications.
Emanueli C, Shearn AIU, Angelini GD, et al., 2015, Exosomes and exosomal miRNAs in cardiovascular protection and repair, VASCULAR PHARMACOLOGY, Vol: 71, Pages: 24-30, ISSN: 1537-1891
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- Citations: 176
Emanueli C, Angelini GD, 2015, Bridging basic science with cardiac surgery: the Bristol Heart Institute experience, Frontiers in Surgery, Vol: 2, ISSN: 2296-875X
Beltrami C, Shantikumar S, Shearn A, et al., 2015, The Human Pericardial Fluid is Enriched With Cardiovascular-expressed Micrornas and Exosomes Able to Elicit Therapeutic Responses, American-Stroke-Association/American-Heart-Association Basic Cardiovascular Sciences Scientific Sessions - Pathways to Cardiovascular Therapeutics, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7330
Aday S, Besnier M, Zoldan J, et al., 2015, MicroRNA 17 in Angiogenesis: Lessons Learned From Immobilized Vascular Endothelial Growth Factor, American-Stroke-Association/American-Heart-Association Basic Cardiovascular Sciences Scientific Sessions - Pathways to Cardiovascular Therapeutics, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7330
Angelini TG, Emanueli C, 2015, MicroRNAs as clinical biomarkers?, FRONTIERS IN GENETICS, Vol: 6, ISSN: 1664-8021
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- Citations: 11
Caputo M, Saif J, Rajakaruna C, et al., 2015, MicroRNAs in vascular tissue engineering and post-ischemic neovascularization, ADVANCED DRUG DELIVERY REVIEWS, Vol: 88, Pages: 78-91, ISSN: 0169-409X
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- Citations: 24
Besnier M, Meloni M, Caporali A, et al., 2015, IL-33/ST2 IN ANGIOGENESIS AND LIMB ISCHEMIA IN MICE, 83rd Congress of the European-Atherosclerosis-Society (EAS), Publisher: ELSEVIER IRELAND LTD, Pages: E22-E22, ISSN: 0021-9150
Avolio E, Rodriguez-Arabaolaza I, Spencer HL, et al., 2015, Expansion and Characterization of Neonatal Cardiac Pericytes Provides a Novel Cellular Option for Tissue Engineering in Congenital Heart Disease, JOURNAL OF THE AMERICAN HEART ASSOCIATION, Vol: 4, ISSN: 2047-9980
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- Citations: 54
Avolio E, Meloni M, Spencer HL, et al., 2015, Combined Intramyocardial Delivery of Human Pericytes and Cardiac Stem Cells Additively Improves the Healing of Mouse Infarcted Hearts Through Stimulation of Vascular and Muscular Repair, CIRCULATION RESEARCH, Vol: 116, Pages: E81-E94, ISSN: 0009-7330
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- Citations: 101
Ascione R, Rowlinson J, Avolio E, et al., 2015, Migration towards SDF-1 selects angiogenin-expressing bone marrow monocytes endowed with cardiac reparative activity in patients with previous myocardial infarction, STEM CELL RESEARCH & THERAPY, Vol: 6
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- Citations: 11
Duggirala A, Delogu F, Angelini TG, et al., 2015, Non coding RNAs in aortic aneurysmal disease, FRONTIERS IN GENETICS, Vol: 6, ISSN: 1664-8021
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- Citations: 30
Gubernator M, Slater SC, Spencer HL, et al., 2015, Epigenetic Profile of Human Adventitial Progenitor Cells Correlates With Therapeutic Outcomes in a Mouse Model of Limb Ischemia, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 35, Pages: 675-688, ISSN: 1079-5642
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- Citations: 32
Floris I, Descamps B, Vardeu A, et al., 2015, Gestational Diabetes Mellitus Impairs Fetal Endothelial Cell Functions Through a Mechanism Involving MicroRNA-101 and Histone Methyltransferase Enhancer of Zester Homolog-2, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 35, Pages: 664-674, ISSN: 1079-5642
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- Citations: 86
Howard L, Caporali A, Shantikumar S, et al., 2015, MicroRNAs in Vascular Remodeling and Repair, MicroRNA in Regenerative Medicine, Pages: 601-629, ISBN: 9780124055445
At the heart of vascular remodeling is a complex network of temporal gene expression coordinated by microRNAs, or miRNAs. Cardiovascular regeneration the generation of new functional cardiomyocytes and therapeutic angiogenesis remains a key goal in cell therapy for vascular and cardiovascular diseases. Cell differentiation elicits a complex concerto of precise miRNA expression for controlling cell fate decisions, function, and engraftment into the host. Limited improvements in animal models of ischemic disease using progenitor cell types have been shown in diseases such as chronic angina, acute MI, and heart failure. These therapies are by no means optimal. Before they can progress to the clinic, more understanding of the transcriptional control of lineage commitment is warranted. This chapter describes the role of microRNAs in vascular remodeling and in cell therapy strategies for treating ischemic diseases.
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