Imperial College London
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Professor Costanza Emanueli

Faculty of Medicine, National Heart & Lung Institute

Chair in Cardiovascular Science
 
 
 
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Contact

 

c.emanueli Website

 
 
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Location

 

434ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Aday:2017:10.1038/s41467-017-00746-7,
author = {Aday, S and Zoldan, J and Besnier, M and Carreto, L and Saif, J and Fernandes, R and Santos, T and Bernardino, L and Langer, R and Emanueli, C and Ferreira, L},
doi = {10.1038/s41467-017-00746-7},
journal = {NATURE COMMUNICATIONS},
pages = {1--14},
title = {Synthetic microparticles conjugated with VEGF(165) improve the survival of endothelial progenitor cells via microRNA-17 inhibition},
url = {http://dx.doi.org/10.1038/s41467-017-00746-7},
volume = {8},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Several cell-based therapies are under pre-clinical and clinical evaluation for the treatment of ischemic diseases. Poor survival and vascular engraftment rates of transplanted cells force them to work mainly via time-limited paracrine actions. Although several approaches, including the use of soluble vascular endothelial growth factor (sVEGF)—VEGF165, have been developed in the last 10 years to enhance cell survival, they showed limited efficacy. Here, we report a pro-survival approach based on VEGF-immobilized microparticles (VEGF-MPs). VEGF-MPs prolong VEGFR-2 and Akt phosphorylation in cord blood-derived late outgrowth endothelial progenitor cells (OEPCs). In vivo, OEPC aggregates containing VEGF-MPs show higher survival than those treated with sVEGF. Additionally, VEGF-MPs decrease miR-17 expression in OEPCs, thus increasing the expression of its target genes CDKN1A and ZNF652. The therapeutic effect of OEPCs is improved in vivo by inhibiting miR-17. Overall, our data show an experimental approach to improve therapeutic efficacy of proangiogenic cells for the treatment of ischemic diseases.
AU  - Aday,S
AU  - Zoldan,J
AU  - Besnier,M
AU  - Carreto,L
AU  - Saif,J
AU  - Fernandes,R
AU  - Santos,T
AU  - Bernardino,L
AU  - Langer,R
AU  - Emanueli,C
AU  - Ferreira,L
DO  - 10.1038/s41467-017-00746-7
EP  - 14
PY  - 2017///
SN  - 2041-1723
SP  - 1
TI  - Synthetic microparticles conjugated with VEGF(165) improve the survival of endothelial progenitor cells via microRNA-17 inhibition
T2  - NATURE COMMUNICATIONS
UR  - http://dx.doi.org/10.1038/s41467-017-00746-7
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000412052400014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/s41467-017-00746-7
UR  - http://hdl.handle.net/10044/1/64454
VL  - 8
ER  -
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