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@article{Abdul-Ghani:2022:10.3892/ijmm.2022.5133,
author = {Abdul-Ghani, S and Skeffington, KL and Kim, M and Moscarelli, M and Lewis, PA and Heesom, K and Fiorentino, F and Emanueli, C and Reeves, BC and Punjabi, PP and Angelini, GD and Suleiman, M-S},
doi = {10.3892/ijmm.2022.5133},
journal = {International Journal of Molecular Medicine},
pages = {1--14},
title = {Effect of cardioplegic arrest and reperfusion on left and right ventricular proteome/phosphoproteome in patients undergoing surgery for coronary or aortic valve disease},
url = {http://dx.doi.org/10.3892/ijmm.2022.5133},
volume = {49},
year = {2022}
}

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TY  - JOUR
AB  - Our earlier work has shown interdisease and intradisease differences in the cardiac proteome between right (RV) and left (LV) ventricles of patients with aortic valve stenosis (AVS) or coronary artery disease (CAD). Whether disease remodeling also affects acute changes occuring in the proteome during surgical intervention is unknown. This study investigated the effects of cardioplegic arrest on cardiac proteins/phosphoproteins in LV and RV of CAD (n=6) and AVS (n=6) patients undergoing cardiac surgery. LV and RV biopsies were collected during surgery before ischemic cold blood cardioplegic arrest (pre) and 20 min after reperfusion (post). Tissues were snap frozen, proteins extracted, and the extracts were used for proteomic and phosphoproteomic analysis using Tandem Mass Tag (TMT) analysis. The results were analysed using QuickGO and Ingenuity Pathway Analysis softwares. For each comparision, our proteomic analysis identified more than 3,000 proteins which could be detected in both the pre and Post samples. Cardioplegic arrest and reperfusion were associated with significant differential expression of 24 (LV) and 120 (RV) proteins in the CAD patients, which were linked to mitochondrial function, inflammation and cardiac contraction. By contrast, AVS patients showed differential expression of only 3 LV proteins and 2 RV proteins, despite a significantly longer duration of ischaemic cardioplegic arrest. The relative expression of 41 phosphoproteins was significantly altered in CAD patients, with 18 phosphoproteins showing altered expression in AVS patients. Inflammatory pathways were implicated in the changes in phosphoprotein expression in both groups. Interdisease comparison for the same ventricular chamber at both timepoints revealed differences relating to inflammation and adrenergic and calcium signalling. In conclusion, the present study found that ischemic arrest and reperfusion trigger different changes in the proteomes and phosphoproteomes of LV and RV of
AU  - Abdul-Ghani,S
AU  - Skeffington,KL
AU  - Kim,M
AU  - Moscarelli,M
AU  - Lewis,PA
AU  - Heesom,K
AU  - Fiorentino,F
AU  - Emanueli,C
AU  - Reeves,BC
AU  - Punjabi,PP
AU  - Angelini,GD
AU  - Suleiman,M-S
DO  - 10.3892/ijmm.2022.5133
EP  - 14
PY  - 2022///
SN  - 1107-3756
SP  - 1
TI  - Effect of cardioplegic arrest and reperfusion on left and right ventricular proteome/phosphoproteome in patients undergoing surgery for coronary or aortic valve disease
T2  - International Journal of Molecular Medicine
UR  - http://dx.doi.org/10.3892/ijmm.2022.5133
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000795884200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://www.spandidos-publications.com/10.3892/ijmm.2022.5133
UR  - http://hdl.handle.net/10044/1/101069
VL  - 49
ER  -

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