Imperial College London
Portrait Picture

Professor Costanza Emanueli

Faculty of Medicine, National Heart & Lung Institute

Chair in Cardiovascular Science
 
 
 
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Contact

 

c.emanueli Website

 
 
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Location

 

434ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Beltrami:2017:10.1016/j.ymthe.2016.12.022,
author = {Beltrami, C and Besnier, M and Shantikumar, S and Shearn, AIU and Rajakaruna, C and Laftah, A and Sessa, F and Spinetti, G and Petretto, E and Angelini, GD and Emanueli, C},
doi = {10.1016/j.ymthe.2016.12.022},
journal = {Molecular Therapy},
pages = {679--693},
title = {Human pericardial fluid contains exosomes enriched with cardiovascular-expressed microRNAs and promotes therapeutic angiogenesis},
url = {http://dx.doi.org/10.1016/j.ymthe.2016.12.022},
volume = {25},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The pericardial fluid (PF) is contained in the pericardial sac surrounding the heart. MicroRNA (miRNA) exchange via exosomes (endogenous nanoparticles) contributes to cell-to-cell communication. We investigated the hypotheses that the PF is enriched with miRNAs secreted by the heart and that it mediates vascular responses through exosome exchange of miRNAs. The study was developed using leftover material from aortic valve surgery. We found that in comparison with peripheral plasma, the PF contains exosomes enriched with miRNAs co-expressed in patients’ myocardium and vasculature. At a functional level, PF exosomes improved survival, proliferation, and networking of cultured endothelial cells (ECs) and restored the angiogenic capacity of ECs depleted (via Dicer silencing) of their endogenous miRNA content. Moreover, PF exosomes improved post-ischemic blood flow recovery and angiogenesis in mice. Mechanistically, (1) let-7b-5p is proangiogenic and inhibits its target gene, TGFBR1, in ECs; (2) PF exosomes transfer a functional let-7b-5p to ECs, thus reducing their TGFBR1 expression; and (3) let-7b-5p depletion in PF exosomes impairs the angiogenic response to these nanoparticles. Collectively, our data support the concept that PF exosomes orchestrate vascular repair via miRNA transfer.
AU  - Beltrami,C
AU  - Besnier,M
AU  - Shantikumar,S
AU  - Shearn,AIU
AU  - Rajakaruna,C
AU  - Laftah,A
AU  - Sessa,F
AU  - Spinetti,G
AU  - Petretto,E
AU  - Angelini,GD
AU  - Emanueli,C
DO  - 10.1016/j.ymthe.2016.12.022
EP  - 693
PY  - 2017///
SN  - 1525-0016
SP  - 679
TI  - Human pericardial fluid contains exosomes enriched with cardiovascular-expressed microRNAs and promotes therapeutic angiogenesis
T2  - Molecular Therapy
UR  - http://dx.doi.org/10.1016/j.ymthe.2016.12.022
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000396635500012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/71849
VL  - 25
ER  -
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