Publications

BibTex format

@article{Alvino:2018:10.1161/JAHA.117.006727,
author = {Alvino, VV and Fernandez-Jimenez, R and Rodriguez-Arabaolaza, I and Slater, S and Mangialardi, G and Avolio, E and Spencer, H and Culliford, L and Hassan, S and Sueiro, Ballesteros L and Herman, A and Ayaon-Albarran, A and Galan-Arriola, C and Sanchez-Gonzalez, J and Hennessey, H and Delmege, C and Ascione, R and Emanueli, C and Angelini, GD and Ibanez, B and Madeddu, P},
doi = {10.1161/JAHA.117.006727},
journal = {Journal of the American Heart Association : Cardiovascular and Cerebrovascular Disease},
title = {Transplantation of allogeneic pericytes improves myocardial vascularization and reduces interstitial fibrosis in a swine model of reperfused acute myocardial infarction},
url = {http://dx.doi.org/10.1161/JAHA.117.006727},
volume = {7},
year = {2018}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundTransplantation of adventitial pericytes (APCs) promotes cardiac repair in murine models of myocardial infarction. The aim of present study was to confirm the benefit of APC therapy in a large animal model.Methods and ResultsWe performed a blind, randomized, placebocontrolled APC therapy trial in a swine model of reperfused myocardial infarction. A first study used human APCs (hAPCs) from patients undergoing coronary artery bypass graft surgery. A second study used allogeneic swine APCs (sAPCs). Primary end points were (1) ejection fraction as assessed by cardiac magnetic resonance imaging and (2) myocardial vascularization and fibrosis as determined by immunohistochemistry. Transplantation of hAPCs reduced fibrosis but failed to improve the other efficacy end points. Incompatibility of the xenogeneic model was suggested by the occurrence of a cytotoxic response following in vitro challenge of hAPCs with swine spleen lymphocytes and the failure to retrieve hAPCs in transplanted hearts. We next considered sAPCs as an alternative. Flow cytometry, immunocytochemistry, and functional/cytotoxic assays indicate that sAPCs are a surrogate of hAPCs. Transplantation of allogeneic sAPCs benefited capillary density and fibrosis but did not improve cardiac magnetic resonance imaging indices of contractility. Transplanted cells were detected in the border zone.ConclusionsImmunologic barriers limit the applicability of a xenogeneic swine model to assess hAPC efficacy. On the other hand, we newly show that transplantation of allogeneic sAPCs is feasible, safe, and immunologically acceptable. The approach induces proangiogenic and antifibrotic benefits, though these effects were not enough to result in functional improvements.
AU  - Alvino,VV
AU  - Fernandez-Jimenez,R
AU  - Rodriguez-Arabaolaza,I
AU  - Slater,S
AU  - Mangialardi,G
AU  - Avolio,E
AU  - Spencer,H
AU  - Culliford,L
AU  - Hassan,S
AU  - Sueiro,Ballesteros L
AU  - Herman,A
AU  - Ayaon-Albarran,A
AU  - Galan-Arriola,C
AU  - Sanchez-Gonzalez,J
AU  - Hennessey,H
AU  - Delmege,C
AU  - Ascione,R
AU  - Emanueli,C
AU  - Angelini,GD
AU  - Ibanez,B
AU  - Madeddu,P
DO  - 10.1161/JAHA.117.006727
PY  - 2018///
SN  - 2047-9980
TI  - Transplantation of allogeneic pericytes improves myocardial vascularization and reduces interstitial fibrosis in a swine model of reperfused acute myocardial infarction
T2  - Journal of the American Heart Association : Cardiovascular and Cerebrovascular Disease
UR  - http://dx.doi.org/10.1161/JAHA.117.006727
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000426642400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/66309
VL  - 7
ER  -

Download

Professor Costanza Emanueli

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)