Imperial College London
Alternate

DrClaireHiggins

Faculty of Engineering, Department of Bioengineering

Reader in Tissue Regeneration
 
 
 
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Contact

 

c.higgins Website

 
 
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Location

 

Uren 319Sir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liu:2018:2018/8631432,
author = {Liu, J and Higgins, C and Whitehouse, C and Crawford, H and Harris, S and Christiano, A and Lako, M and Hole, N and Jahoda, C},
doi = {2018/8631432},
journal = {Stem Cells International},
title = {Hair follicle dermal cells support expansion of murine and human embryonic and induced pluripotent stem cells, and promote haematopoiesis in mouse cultures},
url = {http://dx.doi.org/10.1155/2018/8631432},
volume = {2018},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintainproliferation and differentiation of the epithelial stem cells that produce the hair fibre. In viewof their regulatory properties, in this study we investigated the interaction between hair follicledermal cells (DP and DS) and embryonic stem cells (ESCs), induced pluripotent stem cells(iPSCs) and haematopoietic stem cells. We found that co-culture of follicular dermal cells withESCs or iPSCs supported their prolonged maintenance in an apparently undifferentiated stateas established by differentiation assays, immunocytochemistry and RT-PCR for markers ofundifferentiated ESCs. We further showed that cytokines that are involved in ESC support arealso expressed by cultured follicle dermal cells, providing a possible explanation formaintenance of ES cell stemness in co-cultures. The same cytokines were expressed withinfollicles in situ in a pattern more consistent with a role in follicle growth activities than stem cellmaintenance. Finally we show that cultured mouse follicle dermal cells provide good stromalsupport for haematopoiesis in an established co-culture model. Human follicular dermal cellsrepresent an accessible and readily propagated source of feeder cells for pluripotent andhaematopoietic cells, and have potential for use in clinical applications.
AU  - Liu,J
AU  - Higgins,C
AU  - Whitehouse,C
AU  - Crawford,H
AU  - Harris,S
AU  - Christiano,A
AU  - Lako,M
AU  - Hole,N
AU  - Jahoda,C
DO  - 2018/8631432
PY  - 2018///
SN  - 1687-9678
TI  - Hair follicle dermal cells support expansion of murine and human embryonic and induced pluripotent stem cells, and promote haematopoiesis in mouse cultures
T2  - Stem Cells International
UR  - http://dx.doi.org/10.1155/2018/8631432
UR  - http://hdl.handle.net/10044/1/59297
VL  - 2018
ER  -
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