Imperial College London
Alternate

DrChristopherJohn

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Reader in Pharmacology
 
 
 
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Contact

 

+44 (0)20 3313 8039c.john

 
 
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Location

 

6N5cCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Basharat:2014:10.1530/JOE-13-0249,
author = {Basharat, S and Parker, JA and Murphy, KG and Bloom, SR and Buckingham, JC and John, CD},
doi = {10.1530/JOE-13-0249},
journal = {Journal of Endocrinology},
pages = {229--234},
title = {Leptin fails to blunt the lipopolysaccharide-induced activation of the hypothalamic-pituitary- adrenal axis in rats},
url = {http://dx.doi.org/10.1530/JOE-13-0249},
volume = {221},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Obesity is a risk factor for sepsis morbidity and mortality, whereas the hypothalamic–pituitary–adrenal (HPA) axis plays a protective role in the body's defence against sepsis. Sepsis induces a profound systemic immune response and cytokines serve as excellent markers for sepsis as they act as mediators of the immune response. Evidence suggests that the adipokine leptin may play a pathogenic role in sepsis. Mouse endotoxaemic models present with elevated leptin levels and exogenously added leptin increased mortality whereas human septic patients have elevated circulating levels of the soluble leptin receptor (Ob-Re). Evidence suggests that leptin can inhibit the regulation of the HPA axis. Thus, leptin may suppress the HPA axis, impairing its protective role in sepsis. We hypothesised that leptin would attenuate the HPA axis response to sepsis. We investigated the direct effects of an i.p. injection of 2mg/kg leptin on the HPA axis response to intraperitoneally injected 25μg/kg lipopolysaccharide (LPS) in the male Wistar rat. We found that LPS potently activated the HPA axis, as shown by significantly increased plasma stress hormones, ACTH and corticosterone, and increased plasma interleukin 1β (IL1β) levels, 2h after administration. Pre-treatment with leptin, 2h before LPS administration, did not influence the HPA axis response to LPS. In turn, LPS did not affect plasma leptin levels. Our findings suggest that leptin does not influence HPA function or IL1β secretion in a rat model of LPS-induced sepsis, and thus that leptin is unlikely to be involved in the acute-phase endocrine response to bacterial infection in rats.
AU  - Basharat,S
AU  - Parker,JA
AU  - Murphy,KG
AU  - Bloom,SR
AU  - Buckingham,JC
AU  - John,CD
DO  - 10.1530/JOE-13-0249
EP  - 234
PY  - 2014///
SN  - 0022-0795
SP  - 229
TI  - Leptin fails to blunt the lipopolysaccharide-induced activation of the hypothalamic-pituitary- adrenal axis in rats
T2  - Journal of Endocrinology
UR  - http://dx.doi.org/10.1530/JOE-13-0249
UR  - http://hdl.handle.net/10044/1/30500
VL  - 221
ER  -
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